柑橘红螨,柑橘全甲,是世界上最臭名昭著和最具破坏性的柑橘害虫之一,已经对多种化学杀螨剂产生了抗性。在以前的研究中,我们发现螺氯芬抗性与P450、CCE、和ABC转运蛋白基因。然而,这些解毒基因的调控机制仍然难以捉摸。本研究鉴定了香茅的所有激素受体96个基因。8个PcHR96基因含有高度保守的结构域。表达谱显示,PcHR96h在螺双氯芬抗性菌株中和暴露于螺双氯芬后显著上调。PcHR96h的RNA干扰降低了脱毒基因的表达,并增加了西尼螺旋藻的敏感性。此外,分子对接,异源表达,和药物亲和力响应靶标的稳定性表明,PcHR96h可以与螺氯芬在体外相互作用。我们的研究结果表明,PcHR96h在调节螺氯芬的敏感性中起着重要作用,并为柑橘的耐药性管理提供了理论支持。
The citrus red mite, Panonychus citri, is one of the most notorious and devastating citrus pests around the world that has developed resistance to multiple chemical acaricides. In previous research, we found that spirodiclofen-resistant is related to overexpression of P450, CCE, and ABC transporter genes in P. citri. However, the regulatory mechanisms of these detoxification genes are still elusive. This study identified all hormone receptor 96 genes of P. citri. 8 PcHR96 genes contained highly conserved domains. The expression profiles showed that PcHR96h was significantly upregulated in spirodiclofen resistant strain and after exposure to spirodiclofen. RNA interference of PcHR96h decreased expression of detoxification genes and increased spirodiclofen susceptibility in P. citri. Furthermore, molecular docking, heterologous expression, and drug affinity responsive target stability demonstrated that PcHR96h can interact with spirodiclofen in vitro. Our research results indicate that PcHR96h plays an important role in regulating spirodiclofen susceptibility and provides theoretical support for the resistance management of P. citri.