背景:2022年,全球爆发猴痘,其流行病学特征发生了显着变化。猴痘病毒(MPXV)属于B.1谱系,在这项研究中,研究了与疫情相关的基因组变异。先前的研究表明,病毒基因组变异在病毒的致病性和传播性中起着至关重要的作用。因此,了解MPXV的基因组变异对于控制未来的疫情至关重要.
方法:本研究采用生物信息学和系统发育方法来评估MPXVB.1谱系中的关键基因组变异。共筛选出979株MPXV,分析212个代表性菌株以鉴定病毒基因组中的特异性取代。基于每个位点处最常见的核苷酸,为10个谱系中的每一个构建参考序列。总共鉴定了49个替换,有23个非同义替换。I类变体,对可能影响病毒特性的蛋白质构象有显著影响,被归类为非同义替换。
结果:系统发育分析揭示了10个相对单系的分支。该研究确定了B.1谱系特有的49个替换,有23个非同义替换被归类为I类,II,和III变体。I类变体可能是2022年观察到的循环MPXV特征变化的原因。这些关键突变,特别是I类变体,在MPXV的致病性和传播性中起着至关重要的作用。
结论:这项研究提供了对与最近爆发猴痘相关的B.1谱系中MPXV基因组变异的理解。关键突变的鉴定,特别是I类变体,揭示了观察到的循环MPXV特征变化的分子机制。进一步的研究可以集中在受这些突变影响的功能域上,能够制定有效的控制策略来应对未来的猴痘疫情。
In 2022, a global outbreak of monkeypox occurred with a significant shift in its epidemiological characteristics. The monkeypox virus (MPXV) belongs to the B.1 lineage, and its genomic variations that were linked to the outbreak were investigated in this study. Previous studies have suggested that viral genomic variation plays a crucial role in the pathogenicity and transmissibility of viruses. Therefore, understanding the genomic variation of MPXV is crucial for controlling future outbreaks.
This study employed bioinformatics and phylogenetic approaches to evaluate the key genomic variation in the B.1 lineage of MPXV. A total of 979 MPXV strains were screened, and 212 representative strains were analyzed to identify specific substitutions in the viral genome. Reference sequences were constructed for each of the 10 lineages based on the most common nucleotide at each site. A total of 49 substitutions were identified, with 23 non-synonymous substitutions. Class I variants, which had significant effects on protein conformation likely to affect viral characteristics, were classified among the non-synonymous substitutions.
The phylogenetic analysis revealed 10 relatively monophyletic branches. The study identified 49 substitutions specific to the B.1 lineage, with 23 non-synonymous substitutions that were classified into Class I, II, and III variants. The Class I variants were likely responsible for the observed changes in the characteristics of circulating MPXV in 2022. These key mutations, particularly Class I variants, played a crucial role in the pathogenicity and transmissibility of MPXV.
This study provides an understanding of the genomic variation of MPXV in the B.1 lineage linked to the recent outbreak of monkeypox. The identification of key mutations, particularly Class I variants, sheds light on the molecular mechanisms underlying the observed changes in the characteristics of circulating MPXV. Further studies can focus on functional domains affected by these mutations, enabling the development of effective control strategies against future monkeypox outbreaks.