未经证实:最近的研究证实,长链非编码RNA(lncRNAs)的失调是结肠癌发展和进展的潜在因素。然而,这些RNA分子的预后价值仍存在争议.
UNASSIGNED:本研究旨在研究结肠癌中牛磺酸上调基因-1(TUG1)lncRNA的表达及其临床意义。
未经评估:对47例福尔马林固定,完成手术切除的原发性结肠癌标本的石蜡包埋样本。通过实时逆转录-聚合酶链反应(qRT-PCR)定量从结肠癌样品和非癌邻近组织切片纯化的总RNA,以评估标准化为GAPDH内源性对照的TUG1相对表达水平。此外,采用计算机数据分析。
UNASSIGNED:使用LIVAK方法计算相对表达水平。使用Kaplan-Meier曲线和Cox比例模型评估生存率。P<0.05被认为具有统计学意义。
UNASSIGNED:TUG1在结肠癌标本中的表达相对于非癌组织显著过表达(中位数=21.50,四分位距[IQR]:7.0-209.2;P=0.001)。计算机模拟分析证实结肠癌中TUG1上调(中位数=13.92,IQR:13.5-1432)。TUG1表达与临床病理特征之间无显著关联,如网站,grade,舞台,组织病理学类型,或淋巴管浸润和复发率。同样,Kaplan-Meir和Cox多变量回归分析显示TUG1表达不能预测我们人群中结肠癌患者的总生存期和无进展生存期。
UNASSIGNED:该研究证实了结肠癌组织中TUG1lncRNA的过表达。需要更大的样本量来进一步阐明TUG1在结肠癌中的具体作用。
UNASSIGNED: Recent studies confirmed that dysregulation of long noncoding RNAs (lncRNAs) is a potential contributor to the development and progression of colon cancer. However, the prognostic value of these RNA molecules remains controversial.
UNASSIGNED: This study aimed to investigate the expression of taurine-upregulated gene-1 (TUG1) lncRNA in colon cancer and its clinical implications.
UNASSIGNED: A retrospective study on 47 formalin-fixed, paraffin-embedded samples of surgically resected primary colon cancer specimens was done. Total RNA purified from the colon cancer samples and noncancer adjacent tissue sections was quantified by real-time reverse transcription-polymerase chain reaction (qRT-PCR) to assess TUG1 relative expression levels normalized to GAPDH endogenous control. Also, in silico data analysis was applied.
UNASSIGNED: The relative expression levels were calculated using the LIVAK method. The survival rates were assessed using the Kaplan-Meier curves and the Cox proportional model. P < 0.05 was considered statistically significant.
UNASSIGNED: TUG1expression in the colon cancer specimens was significantly overexpressed (median = 21.50, interquartile range [IQR]: 7.0-209.2; P = 0.001) relative to the noncancerous tissues. In silico analysis confirmed TUG1 upregulation in colon carcinoma (median = 13.92, IQR: 13.5-1432). There were no significant associations between TUG1 expression and clinicopathological characteristics, such as the site, grade, stage, histopathological type, or the rates of lymphovascular invasion and relapse. Similarly, Kaplan-Meir and Cox multivariate regression analyses showed that TUG1 expression could not predict the overall survival and progression-free survival in colon cancer patients of our population.
UNASSIGNED: This study confirms the overexpression of TUG1 lncRNA in colon cancer tissues. Larger sample size is warranted to further elucidate the specific role of TUG1 in colon cancer.