■慢性萎缩性胃炎(CAG)是一种由多种因素引起的复杂慢性疾病,在临床上经常发生。CAG的全球患病率很高。有趣的是,临床CAG患者常表现出多种症状表型,这使得临床医生更难治疗。因此,迫切需要提高我们对临床CAG人群复杂性的认识,获得更准确的疾病亚型,探讨临床症状与用药的关系。因此,基于复杂网络和临床研究的集成平台,我们根据不同的临床特征对所收集的CAG患者进行分类,并对分类结果进行相关性分析,以识别更准确的疾病亚型,从而有助于个性化的临床治疗.
■中医(TCM)提供了复杂疾病的临床亚型的经验理解,因为中医治疗是针对患者的症状特征而定制的。我们收集了6,253份来自CAG患者的中医临床电子病历(EMR),并进行了手动注释,提取,并对数据进行了预处理。创建了共享的症状-患者相似性网络(PSN)。建立CAG患者亚组,并通过采用社区识别方法的富集分析确定其临床特征。根据识别症状-植物本源药物对应的有效性,对相关亚组的不同临床特征进行关联。此外,使用基因本体论(GO)和京都基因和基因组百科全书(KEGG)途径富集分析,网络药理学用于鉴定筛选的症状和药物之间可能的生物学关系,并鉴定关键亚型的各种临床和分子方面.
■5,132名患者被纳入研究:2,699名男性(52.60%)和2,433名女性(47.41%)。人口分为176个模块。我们选择了前3个模块(M29,M3和M0)来说明CAG疾病亚型的特征表型和基因型。M29亚组的特征是胃胀性疾病和浑浊和中毒的内部综合征。M3亚组的特征是上腹痛和肝胃不和。M0亚组的特征是上腹痛和湿热证。在症状分析中,在所有三个亚组中,症状改善的首要症状是胃痛,腹胀,失眠,食欲不振,和胃灼热。然而,三组不同。M29亚组更有可能出现胃胀,厌食症,还有心悸.柑橘医生,龙葵,江坎,山慈蘑菇,狄龙是最受欢迎的植物药。M3亚组黄色尿的发生率较高,苦涩的舌头,还有胃痛.Smilaxglabra,圆形香附,当归,Conioselinumanthriscoides,白芍是使用的植物性药物。呕吐,恶心,胃痛,食欲减退在M0亚组很常见。主要用药是黄芩,Smilaxglabra,Picrorhizakurroa,蓝叶百合,和沙蒿。通过GO和KEGG通路分析,我们发现在M29亚组中,柑橘医生,龙葵,江坎,山慈蘑菇,狄龙可能会对胃胀症状发挥治疗作用,厌食症,和心悸通过调节细胞凋亡和NF-κB信号通路。在M3子群中,Smilaxglabra,圆形香附,当归,Conioselinumanthriscoides,和赤芍可能通过NF-κB和JAK-STAT信号通路治疗胃痛,苦涩的嘴,黄色的尿液在M0子组中,黄芩,Smilaxglabra,Picrorhizakurroa,蓝叶百合,和蒿可能对食欲不振发挥治疗作用,胃痛,呕吐,和恶心通过PI3K-Akt信号通路。
■基于PSN识别和社区检测分析,CAG人群划分可以为临床CAG医治供给有用的建议。该方法可用于CAG疾病分类和基因分型研究,可用于其他复杂的慢性疾病。
UNASSIGNED: Chronic atrophic gastritis (CAG) is a complex chronic disease caused by multiple factors that frequently occurs disease in the clinic. The worldwide prevalence of CAG is high. Interestingly, clinical CAG patients often present with a variety of symptom phenotypes, which makes it more difficult for clinicians to treat. Therefore, there is an urgent need to improve our understanding of the complexity of the clinical CAG population, obtain more accurate disease subtypes, and explore the relationship between clinical symptoms and medication. Therefore, based on the integrated platform of complex networks and clinical research, we classified the collected patients with CAG according to their different clinical characteristics and conducted correlation analysis on the classification results to identify more accurate disease subtypes to aid in personalized clinical treatment.
UNASSIGNED: Traditional Chinese medicine (TCM) offers an empirical understanding of the clinical subtypes of complicated disorders since TCM therapy is tailored to the patient\'s symptom profile. We gathered 6,253 TCM clinical electronic medical records (EMRs) from CAG patients and manually annotated, extracted, and preprocessed the data. A shared symptom-patient similarity network (PSN) was created. CAG patient subgroups were established, and their clinical features were determined through enrichment analysis employing community identification methods. Different clinical features of relevant subgroups were correlated based on effectiveness to identify symptom-botanical botanical drugs correspondence. Moreover, network pharmacology was employed to identify possible biological relationships between screened symptoms and medications and to identify various clinical and molecular aspects of the key subtypes using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis.
UNASSIGNED: 5,132 patients were included in the study: 2,699 males (52.60%) and 2,433 females (47.41%). The population was divided into 176 modules. We selected the first 3 modules (M29, M3, and M0) to illustrate the characteristic phenotypes and genotypes of CAG disease subtypes. The M29 subgroup was characterized by gastric fullness disease and internal syndrome of turbidity and poison. The M3 subgroup was characterized by epigastric pain and disharmony between the liver and stomach. The M0 subgroup was characterized by epigastric pain and dampness-heat syndrome. In symptom analysis, The top symptoms for symptom improvement in all three subgroups were stomach pain, bloating, insomnia, poor appetite, and heartburn. However, the three groups were different. The M29 subgroup was more likely to have stomach distention, anorexia, and palpitations. Citrus medica, Solanum nigrum, Jiangcan, Shan ci mushrooms, and Dillon were the most popular botanical drugs. The M3 subgroup has a higher incidence of yellow urine, a bitter tongue, and stomachaches. Smilax glabra, Cyperus rotundus, Angelica sinensis, Conioselinum anthriscoides, and Paeonia lactiflora were the botanical drugs used. Vomiting, nausea, stomach pain, and appetite loss are common in the M0 subgroup. The primary medications are Scutellaria baicalensis, Smilax glabra, Picrorhiza kurroa, Lilium lancifolium, and Artemisia scoparia. Through GO and KEGG pathway analysis, We found that in the M29 subgroup, Citrus medica, Solanum nigrum, Jiangcan, Shan ci mushrooms, and Dillon may exert their therapeutic effects on the symptoms of gastric distension, anorexia, and palpitations by modulating apoptosis and NF-κB signaling pathways. In the M3 subgroup, Smilax glabra, Cyperus rotundus, Angelica sinensis, Conioselinum anthriscoides, and Paeonia lactiflora may be treated by NF-κB and JAK-STAT signaling pathway for the treatment of stomach pain, bitter mouth, and yellow urine. In the M0 subgroup, Scutellaria baicalensis, Smilax glabra, Picrorhiza kurroa, Lilium lancifolium, and Artemisia scoparia may exert their therapeutic effects on poor appetite, stomach pain, vomiting, and nausea through the PI3K-Akt signaling pathway.
UNASSIGNED: Based on PSN identification and community detection analysis, CAG population division can provide useful recommendations for clinical CAG treatment. This method is useful for CAG illness classification and genotyping investigations and can be used for other complicated chronic diseases.