■反应性口腔病变构成诊断困难,因为它们在临床上相互模仿。根据该组病变的组织病理学表现做出明确的诊断。基质微环境是这些病变阶段顺序的关键。对基质的每个成分的严格定量对于理解发病机理很重要。目的是评估炎症,血管生成,和纤维化的反应性组的病变通过定量分析。
■刺激纤维瘤的块,炎性纤维增生,化脓性肉芽肿,从档案中检索正常粘膜,并进行苏木精和伊红(H&E)和Masson三色染色。炎症的严重程度,上皮厚度,胶原蛋白比例面积,胶原蛋白的综合密度,平均血管面积(MVA),平均血管周长(MVP),和平均血管面积百分比(MBVPA)使用ImageJ软件版本1.8进行定量分析。定性分析了上皮-结缔组织界面处的网状脊的图案。
■炎性纤维增生表现为严重炎症(60%)。化脓性肉芽肿的平均血管面积(MVPA)和平均血管周长(MVP)增加。在刺激性纤维瘤中发现平均胶原蛋白比例面积和胶原蛋白的整合密度更大(64.47%,2519638.01±810471.58μm2)。炎性纤维增生中上皮厚度最高(62.71±18.86μm)。
■反应性口腔病变在组织学上是不同的,然而,根据病变的阶段,它们表现出相当大的重叠。单个致病成分的形态定量探索可能有助于特定诊断。
UNASSIGNED: Reactive oral lesions pose diagnostic difficulties as they mimic each other clinically. A definitive diagnosis is made based on the histopathological presentation of this group of lesion. Stromal microenvironment is the key to the sequence of the stages of these lesions. Stringent quantification of each component of the stroma is important to understand the pathogenesis. The aim is to evaluate inflammation, angiogenesis, and fibrosis in the reactive group of lesions through quantitative analysis.
UNASSIGNED: Blocks of irritation fibroma, inflammatory fibrous hyperplasia, pyogenic granuloma, and normal mucosa were retrieved from the archives and Hematoxylin and Eosin (H&E) and Masson Trichrome staining were done. The severity of inflammation, epithelial thickness, collagen proportionate area, integrated density of collagen, Mean Vascular Area (MVA), Mean Vascular Perimeter (MVP), and Mean blood vessel percentage area (MBVPA) were analysed quantitatively using Image J software version 1.8. The pattern of rete ridges at the epithelium-connective tissue interface was analysed qualitatively.
UNASSIGNED: Inflammatory fibrous hyperplasia presented with severe inflammation (60%). Mean Vascular Percentage Area (MVPA) and Mean Vascular Perimeter (MVP) were increased in pyogenic granuloma. The mean collagen proportionate area and the integrated density of collagen were found to be more in irritation fibroma (64.47%, 2519638.01 ± 810471.58 μm2). The epithelial thickness was highest in inflammatory fibrous hyperplasia (62.71 ± 18.86 μm).
UNASSIGNED: Reactive oral lesions are histologically distinct, yet they exhibit considerable overlap depending on the stage of the lesion. A morphometric quantitative exploration of the individual pathogenic components may aid in specific diagnosis.