磷(P)是一种重要的常量营养素,与raphiopsisraciborskii的生长和增殖有关,一种入侵和臭名昭著的水华形成蓝细菌。然而,对于raciborskii来说,参与P适应的分子机制仍未被探索。这里,进行了raphiopsisraciborskii的转录组测序,以揭示模拟磷酸二钾(DIP)的多方面机制,β-甘油磷酸酯(Gly),2-氨基乙基膦酸(AEP),和无P条件(NP)。与DIP和Gly组相比,NP和AEP组的叶绿素a荧光参数显示出显着差异。磷酸盐运输和摄取相关基因的表达水平,有机磷利用,氮代谢,尿素循环,碳固定,氨基酸代谢,环境信息,糖酵解中的ATP合成过程,三羧酸(TCA)循环,戊糖磷酸途径显著上调,而那些与光合作用有关的,藻胆蛋白,呼吸,氧化磷酸化,硫代谢,与DIP组相比,NP组的遗传信息明显下调。然而,参与有机磷利用的基因表达,尿素循环,和Gly群体的遗传信息,和碳磷裂解酶,与DIP组相比,AEP组的遗传信息和环境信息显着增加。一起,这些结果表明,raphiopsisraciborskii表现出多个代谢途径和某些关键基因的协调进化,以适应环境P的变化,这意味着如果降低P来控制raphiopsisraciborskii开花,外部营养素(如氮,氨基酸,和尿素)将刺激Raphidiopsis的生长或代谢。
Phosphorus (P) is a vital macronutrient associated with the growth and proliferation of Raphidiopsis raciborskii, an invasive and notorious bloom-forming cyanobacterium. However, the molecular mechanisms involved in P acclimation remain largely unexplored for Raphidiopsis raciborskii. Here, transcriptome sequencing of Raphidiopsis raciborskii was conducted to reveal multifaceted mechanisms involved in mimicking dipotassium phosphate (DIP), β-glycerol phosphate (Gly), 2-aminoethylphosphonic acid (AEP), and P-free conditions (NP). Chlorophyll a fluorescence parameters showed significant differences in the NP and AEP groups compared with the DIP and Gly-groups. Expression levels of genes related to phosphate transportation and uptake, organic P utilization, nitrogen metabolism, urea cycling, carbon fixation, amino acid metabolism, environmental information, the ATP-synthesis process in glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway were remarkably upregulated, while those related to photosynthesis, phycobiliproteins, respiration, oxidative phosphorylation, sulfur metabolism, and genetic information were markedly downregulated in the NP group relative to the DIP group. However, the expression of genes involved in organic P utilization, the urea cycle, and genetic information in the Gly-group, and carbon-phosphorus lyase, genetic information and environmental information in the AEP group were significantly increased compared to the DIP group. Together, these results indicate that Raphidiopsis raciborskii exhibits the evolution of coordination of multiple metabolic pathways and certain key genes to adapt to ambient P changes, which implies that if P is reduced to control Raphidiopsis raciborskii bloom, there is a risk that external nutrients (such as nitrogen, amino acids, and urea) will stimulate the growth or metabolism of Raphidiopsis.