背景:建立和验证测试生物样品的方法是药代动力学研究中的关键步骤。目前,关于雷帕霉素血浆浓度的检测,已经发表了一些方法学报告。
目的:本研究的目的是探索检测大鼠全血生物样品中雷帕霉素的有效方法。
方法:在本研究中,我们设计了一个快速的,敏感,和特定的液相色谱-质谱仪/质谱仪(LC-MS/MS)方法检测大鼠全血生物样品中的雷帕霉素。我们全面验证了特异性,线性范围,定量下限(LLOQ),精度,准确度,recovery,和该方法的稳定性。
结果:这项研究的结果证实了LC-MS/MS检测雷帕霉素的成功实施,展示了它的敏感性,特异性,定量分析的可靠性。该方法通过我们验证的LC-MS/MS方法确保了后续研究数据的准确性和可靠性。
结论:结果证明了LC-MS/MS方法的成功实施,具体,和可靠的定量分析大鼠全血样品中雷帕霉素。该方法保证了后续研究数据的准确性和可靠性。
结论:这项研究的重要性在于成功建立了一个快速、敏感,和特异性LC-MS/MS方法检测大鼠全血中雷帕霉素的浓度,确保后续研究数据的准确性和可靠性。这为进一步了解雷帕霉素在体内的代谢和药理作用提供了重要的工具和基础。协助推进相关领域的药物研究和临床应用。
BACKGROUND: The establishment and validation of methods for testing biological samples are crucial steps in pharmacokinetic studies. Currently, several methodological reports have been published on the detection of
rapamycin plasma concentrations.
OBJECTIVE: The objective of this study was to explore an effective method for detecting
rapamycin in rat whole blood biological samples.
METHODS: In this study, we designed a rapid, sensitive, and specific liquid chromatograph-mass spectrometer/mass spectrometer (LC-MS/MS) methodology for detecting
rapamycin in rat whole blood biological samples. We comprehensively validated the specificity, linear range, lower limit of quantification (LLOQ), precision, accuracy, recovery, and stability of this method.
RESULTS: The findings of this study confirmed the successful implementation of LC-MS/MS for the detection of
rapamycin, demonstrating its sensitivity, specificity, and reliability in quantitative analysis. This method ensures the accuracy and reliability of subsequent study data through our validated LC-MS/MS approach.
CONCLUSIONS: The results demonstrated the successful implementation of an LC-MS/MS method for sensitive, specific, and reliable quantitative analysis of
rapamycin in rat whole blood samples. This method ensures the accuracy and reliability of subsequent study data.
CONCLUSIONS: The importance of this study lies in the successful establishment of a rapid, sensitive, and specific LC-MS/MS method for detecting rapamycin concentration in rat whole blood, ensuring the accuracy and reliability of subsequent research data. This provides a crucial tool and foundation for further understanding the metabolism and pharmacological effects of rapamycin in vivo, aiding in the advancement of drug research and clinical applications in related fields.