有可能在战场或恐怖活动中使用化学武器,针对毁灭性眼外伤的有效疗法,从他们的曝光,是需要的。氧气在眼部组织保存和伤口修复中起着至关重要的作用。我们测试了过饱和氧乳剂(SSOE)在减少氯化苦(CP)离体角膜和角膜细胞损伤中的功效。CP,目前用作杀虫剂,是一种化学威胁剂,如芥末毒剂,会导致严重的角膜损伤。由于我们先前在人角膜上皮细胞中的研究显示了SSOE的治疗潜力(55%),我们进一步在离体CP诱导的兔角膜损伤模型中测试了其功效。角膜暴露于CP(700nmol)2小时,在有或没有SSOE的情况下洗涤并培养24小时或96小时。在CP暴露后96小时,SSOE治疗呈现角膜上皮层愈合趋势,并废除了CP诱导的上皮凋亡细胞死亡。SSOE处理还减少了CP诱导的DNA损伤(H2A。X磷酸化)和炎症标志物(例如MMP9,IL-21,MIP-1β,TNFα)。进一步检查SSOE单独或与其他疗法组合在CP和囊泡的体内角膜损伤模型中的治疗功效,是有保证的。
With a possibility for the use of chemical weapons in battlefield or in terrorist activities, effective therapies against the devastating ocular injuries, from their exposure, are needed. Oxygen plays a vital role in ocular tissue preservation and wound repair. We tested the efficacy of supersaturated oxygen emulsion (SSOE) in reducing ex vivo corneal and keratocyte injury from chloropicrin (CP). CP, currently used as a pesticide, is a chemical threat agent like the vesicating mustard agents and causes severe corneal injury. Since our previous study in human corneal epithelial cells showed the treatment potential of SSOE (55 %), we further tested its efficacy in an ex vivo CP-induced rabbit corneal injury model. Corneas were exposed to CP (700 nmol) for 2 h, washed and cultured with or without SSOE for 24 h or 96 h. At 96 h post CP exposure, SSOE treatment presented a healing tendency of the corneal epithelial layer, and abrogated the CP-induced epithelial apoptotic cell death. SSOE treatment also reduced the CP induced DNA damage (H2A.X phosphorylation) and inflammatory markers (e.g. MMP9, IL-21, MIP-1β, TNFα). Further examination of the treatment efficacy of SSOE alone or in combination with other therapies in in vivo cornea injury models for CP and vesicants, is warranted.