■碳纳米管(CNT)是纳米技术中的重要发现,在现代技术中得到广泛应用。然而,人们担心它们的潜在毒性,特别是在皮肤细胞中。本研究旨在探讨碳纳米管诱导小鼠皮肤成纤维细胞细胞毒性和凋亡的机制。
■将小鼠皮肤成纤维细胞分离并暴露于不同浓度的两种类型的CNT,然后分析其活力的变化,活性氧(ROS)的产生,Bcl-2相关X蛋白(Bax)的水平,和乳酸生产。
■结果表明CNT以剂量依赖性方式降低细胞活力并增加ROS产生。此外,目前的研究发现CNTs增加了Bax的蛋白质水平,一种促凋亡蛋白,在小鼠皮肤成纤维细胞中。此外,在暴露于CNT的细胞中观察到乳酸产生的显著降低。
■研究结果得出结论,碳纳米管有可能成为皮肤成纤维细胞的有毒物质,作为身体的第一道防线。这可以通过它们增加ROS产生和Bax蛋白水平的能力来证明,以及降低乳酸水平。据报道,乳酸对皮肤胶原蛋白的产生具有有益作用,需要进一步的研究来充分了解碳纳米管暴露对人体皮肤健康的影响。
UNASSIGNED: Carbon nanotubes (CNTs) are a significant discovery in nanotechnology, with widespread applications in modern technology. However, there are concerns about their potential toxicity, particularly in skin cells. This study aimed to investigate the mechanisms by which CNTs induced cytotoxicity and apoptosis in mouse skin fibroblasts.
UNASSIGNED: The mice skin fibroblasts were isolated and exposed to two types of CNTs at various concentrations and then analyzed for changes in viability, reactive oxygen species (ROS) production, the levels of Bcl-2-associated X protein (Bax), and lactate production.
UNASSIGNED: The results demonstrated that CNTs reduced cell viability and increased ROS production in a dose-dependent manner. Additionally, the current study found that CNTs increased the protein levels of Bax, a pro-apoptotic protein, in mouse skin fibroblasts. Furthermore, it was observed a significant decrease in lactate production in cells exposed to CNTs.
UNASSIGNED: The findings concluded that CNTs have the potential to be toxic substances for skin fibroblasts, which serve as the body\'s first line of defense. This is evidenced by their ability to increase the production of ROS and the protein levels of Bax, as well as reduce lactic acid levels. As lactic acid has been reported to have beneficial effects on skin collagen production, further studies are needed to fully understand the impact of carbon nanotube exposure on human skin health.