结直肠癌在所有癌症类型中发病率第三高,死亡率第二高。探索驱动结直肠癌恶性增殖和转移的分子机制将有利于癌症患者的治疗和管理。最近的研究报道了环指蛋白128(RNF128)在不同类型的癌症中的截然相反的作用。然而,RNF128在结直肠癌中的作用尚不清楚,这项研究试图分析。用RT-qPCR方法检测30对结直肠癌及相应癌周组织中RNF128mRNA和蛋白的差异表达,免疫印迹和免疫组织化学染色。将特异性靶向RNF128的siRNA转染到体外培养的结肠直肠癌细胞系(SW1116和SW480)中。扩散,增长,迁移,通过CCK-8,克隆形成检查结直肠癌细胞的侵袭和上皮间质转化(EMT),伤口愈合,transwell,蛋白质印迹和免疫荧光分析。与癌旁组织相比,结直肠癌组织中的RNF128mRNA和蛋白质水平均显着增加。敲除RNF128显著抑制细胞增殖,增长,迁移,SW480和SW1116细胞的侵袭和EMT。靶向RNF128可能有益于结直肠癌的治疗和管理。
Colorectal cancer has the third highest incidence and second highest mortality rate among all cancer types. Exploring the molecular mechanisms driving malignant proliferation and metastasis of colorectal cancer will benefit the treatment and management of cancer patients. Recent studies have reported diametrically opposed roles of Ring finger protein 128 (
RNF128) in different types of cancer. However, the role of
RNF128 in colorectal cancer is still completely unknown, which this study attempts to analyze. The differential expression of RNF128 mRNA and protein in 30 pairs of colorectal cancer and corresponding peritumoral tissues was detected using RT-qPCR, western blot and immunohistochemical staining. siRNA specifically targeting RNF128 was transfected into colorectal cancer cell lines (SW1116 and SW480) cultured in vitro. Proliferation, growth, migration, invasion and epithelial-mesenchymal transition (EMT) of colorectal cancer cells were examined by CCK-8, clone formation, wound-healing, transwell, western blot and immunofluorescence assays. Both
RNF128 mRNA and protein levels were significantly increased in colorectal cancer tissues compared to pericarcinoma tissues. Knockdown of
RNF128 significantly inhibited the proliferation, growth, migration, invasion and EMT of SW480 and SW1116 cells. Targeting
RNF128 may benefit the treatment and management of colorectal cancer.