目的:探讨RELN启动子区CpG岛甲基化与精神分裂症阳性(I型)和阴性(II型)类型之间的关系。并探讨血清白细胞介素(IL)-1β,精神分裂症中的IL-6和髓磷脂碱性蛋白(MBP)。
方法:检测精神分裂症患者(实验组)和健康个体(对照组)外周血中RELN启动子区CpG岛甲基化水平。和血清IL-1β,测量IL-6和MBP。
结果:实验组RELN启动子区CpG岛甲基化阳性率高于对照组;I型和II型患者之间没有显着差异。阳性和阴性综合征量表(PANSS)评分及血清IL-1β、I型和II型患者之间的IL-6和MBP。此外,血清IL-1β呈正相关,II型患者的IL-6,MBP和PANSS评分(阴性症状)。
结论:RELN启动子区CpG岛甲基化与精神分裂症有关,但不是它的临床类型。I型和II型精神分裂症可能有不同的病理机制,而Ⅱ型精神分裂症可能与血清IL-1β、IL-6和MBP。
OBJECTIVE: To explore the association between CpG island methylation in the promoter region of RELN and positive (type I) and negative (type II) types of schizophrenia, and investigate serum interleukin (IL)-1β, IL-6, and myelin basic protein (MBP) in schizophrenia.
METHODS: Levels of CpG island methylation in the promoter region of RELN were detected in peripheral blood of patients with schizophrenia (experimental group) and healthy individuals (control group), and serum IL-1β, IL-6, and MBP were measured.
RESULTS: The positive rate of CpG island methylation in the promoter region of RELN was higher in the experimental group than in the control group; however, there were no significant differences between type I and II patients. There were differences in Positive and Negative Syndrome Scale (PANSS) scores and serum IL-1β, IL-6, and MBP between type I and II patients. Furthermore, there were positive correlations between serum IL-1β, IL-6, and MBP and PANSS scores (negative symptoms) in type II patients.
CONCLUSIONS: CpG island methylation in the promoter region of RELN was associated with schizophrenia, but not with its clinical type. There may be different pathological mechanisms in type I and II schizophrenia, and type II schizophrenia may be associated with serum IL-1β, IL-6, and MBP.