目的:这项研究的重点是评估从泰国运河水中分离的沙门氏菌菌株中质粒介导的喹诺酮耐药(PMQR)决定因素的患病率和抗菌药物敏感性。
结果:从2016年到2020年,从曼谷的六个运河中收集了333个水样,泰国。沙门氏菌属。被隔离,通过聚合酶链反应检测质粒介导的喹诺酮耐药(PMQR)基因,并使用圆盘扩散法检查抗菌药物敏感性。结果表明沙门氏菌的患病率为92.2%。在运河水中,血清群B和C是最常见的检测。总的来说,35.3%的分离株携带PMQR基因,qnrS是最普遍的基因(97.2%,n=137/141)。其他PMQR基因,包括qnrB,qnrD,OQXAB,并检测到aac(6')-Ib-cr。值得注意的是,六个分离株携带多个PMQR基因。此外,9.3%和3.8%的分离株对萘啶酸(NAL)和环丙沙星(CIP)耐药,分别。PMQR阳性分离株对两种NAL的不敏感率较高(48.2%,n=68/141)和CIP(92.2%,n=130/141)与PMQR阴性分离株(NAL:8.9%,n=23/258;CIP:11.2%,n=30/258)。
结论:沙门氏菌的高患病率。,显著的PMQR阳性,在曼谷,运河水中的敏感性降低是公共卫生问题。
OBJECTIVE: This research focused on assessing the prevalence of plasmid-mediated quinolone resistance (PMQR) determinants and antimicrobial susceptibility in Salmonella strains isolated from Thai canal water.
RESULTS: From 2016 to 2020, 333 water samples were collected from six canals across Bangkok, Thailand. Salmonella spp. was isolated, PMQR genes were detected through polymerase chain reactions, and the antimicrobial susceptibility was examined using the disk diffusion method. The results indicated a 92.2% prevalence of Salmonella spp. in canal water, being serogroups B and C the most frequently detected. Overall, 35.3% of isolates harbored PMQR genes, being qnrS the most prevalent gene (97.2%, n = 137/141). Other PMQR genes, including qnrB, qnrD, oqxAB, and aac(6\')-Ib-cr, were detected. Notably, six isolates harbored multiple PMQR genes. Furthermore, 9.3% and 3.8% of the overall isolates were resistant to nalidixic acid (NAL) and ciprofloxacin (CIP), respectively. PMQR-positive isolates showed higher rates of non-susceptibility to both NAL (48.2%, n = 68/141) and CIP (92.2%, n = 130/141) compared to PMQR-negative isolates (NAL: 8.9%, n = 23/258; CIP: 11.2%, n = 30/258).
CONCLUSIONS: The high prevalence of Salmonella spp., significant PMQR-positive, and reduced susceptibility isolates in canal water is of public health concern in Bangkok.