蛋白质在生物系统中起着重要的作用,几种蛋白质被用于诊断,治疗,食品工业等。因此,有关蛋白质物理性质的知识至关重要,这将有助于理解它们的功能和后续应用。蛋白质的解链温度(Tm)是提供关于蛋白质在不同条件下的稳定性的信息的基本参数之一。在本研究中,我们已经证明了一种使用QuinaldineRed(QR)与蛋白质之间的超分子相互作用确定蛋白质Tm的方法。使用此方法,我们已经确定了5种蛋白质的Tm,并将我们的结果与既定方案进行了比较.我们的结果显示与其他方法和已发表的值具有良好的一致性。在这项研究中开发的方法是廉价的,快,并且没有复杂的仪器和样品的前/后处理。此外,该方法可用于多板模式下的高通量。因此,这项研究计划了一种新的方法,通过用户友好的操作来测定各种蛋白质的Tm。
Proteins play an important role in biological systems and several proteins are used in diagnosis, therapy, food industry etc. Thus, knowledge about the physical properties of the proteins is of utmost importance, which will aid in understanding their function and subsequent applications. The melting temperature (Tm) of a protein is one of the essential parameters which gives information about the stability of a protein under different conditions. In the present study, we have demonstrated a method for determining the Tm of proteins using the supramolecular interaction between Quinaldine Red (QR) and proteins. Using this method, we have determined the Tm of 5 proteins and compared our results with established protocols. Our results showed good agreement with the other methods and published values. The method developed in this study is inexpensive, quick, and devoid of complex instruments and pre/post-treatment of the samples. In addition, this method can be adopted for high throughput in multi-plate mode. Thus, this study projects a new methodology for Tm determination of various proteins with user friendly operation.