Pigeonpea(Cajanuscajan)是半干旱地区的重要作物,也是印度膳食蛋白质的重要来源。该植物对盐度胁迫敏感,这对其生产力产生了不利影响。基于盐度胁迫对木豆幼苗生长和离子含量的剂量依赖性影响,使用7天龄的幼苗进行对照和盐胁迫(150mMNaCl)植物的比较蛋白质组分析。在各种氨基酸中,丝氨酸,天冬氨酸和天冬酰胺是根增加的氨基酸,而丝氨酸,盐胁迫下,天冬氨酸和苯丙氨酸在芽中呈上升趋势。此外,无标签和无凝胶的比较Q-Tof,液相色谱-质谱(LC-MS)显示,在有和没有盐胁迫条件的情况下,根和芽中总共有118种差异丰富的蛋白质。与单指(Dof)转录因子家族和甘氨酸甜菜碱(GB)生物合成的DNA结合相关的蛋白质在盐度胁迫的幼苗的芽和根中差异表达。外源施用胆碱对盐胁迫下GB积累的影响表现为GB途径的增加。差异丰富蛋白的基因表达分析揭示了乙醇胺激酶(CcEthKin)的较高诱导,胆碱-磷酸磷脂酰基转移酶1-样(CcChoPh),丝氨酸羟甲基转移酶(CcSHMT)和Dof蛋白(CcDof29)。结果表明,胆碱前体,盐度胁迫耐受性中的丝氨酸生物合成途径和甘氨酸甜菜碱合成。甘氨酸甜菜碱保护植物免受细胞损伤,并在胁迫条件下充当渗透剂。蛋白质相互作用网络(PIN)分析表明,61%的差异表达蛋白质表现出正相互作用,其中10%形成PIN的中心。Further,PIN分析还强调了细胞色素c氧化酶在控制木豆盐度应激反应的传感和信号级联中的潜在作用。
背景:在线版本包含补充材料,可在10.1007/s12298-021-01116-w获得。
Pigeonpea (Cajanus cajan) is an important crop in semi-arid regions and a significant source of dietary proteins in India. The plant is sensitive to salinity stress, which adversely affects its productivity. Based on the dosage-dependent influence of salinity stress on the growth and ion contents in the young seedlings of pigeonpea, a comparative proteome analysis of control and salt stressed (150 mM NaCl) plants was conducted using 7 days-old seedlings. Among various amino acids, serine, aspartate and asparagine were the amino acids that showed increment in the root, whereas serine, aspartate and phenylalanine showed an upward trend in shoots under salt stress. Furthermore, a label-free and gel-free comparative Q-Tof, Liquid Chromatography-Mass spectrometry (LC-MS) revealed total of 118 differentially abundant proteins in roots and shoots with and without salt stress conditions. Proteins related to DNA-binding with one finger (Dof) transcription factor family and glycine betaine (GB) biosynthesis were differentially expressed in the shoot and root of the salinity-stressed seedlings. Exogenous application of choline on GB accumulation under salt stress showed the increase of GB pathway in C. cajan. Gene expression analysis for differentially abundant proteins revealed the higher induction of ethanolamine kinase (CcEthKin), choline-phosphate cytidylyltransferase 1-like (CcChoPh), serine hydroxymethyltransferase (CcSHMT) and Dof protein (CcDof29). The results indicate the importance of, choline precursor, serine biosynthetic pathways and glycine betaine synthesis in salinity stress tolerance. The glycine betaine protects plant from cellular damages and acts as osmoticum under stress condition. Protein interaction network (PIN) analysis demonstrated that 61% of the differentially expressed proteins exhibited positive interactions and 10% of them formed the center of the PIN. Further, The PIN analysis also highlighted the potential roles of the cytochrome c oxidases in sensing and signaling cascades governing salinity stress responses in pigeonpea.
BACKGROUND: The online version contains supplementary material available at 10.1007/s12298-021-01116-w.