目的:泥腐菌导致一种难以治疗的感染性疾病,称为化脓症,高发病率和死亡率。到目前为止,至少10株品系的基因组数据,主要分类在系统发育分化I和II中,已经使用各种下一代测序平台进行了测序。使用MGI短读平台从泰国和美国的患者中获得了2种落叶杆菌III株(最近被重新分类为周腐霉)的基因组数据。这项工作是我们尝试从各种病原体菌株中生成综合基因组数据库的一部分。
方法:从阴间假单胞菌的gDNA样品中制备了150bp的配对末端文库(P.periculosum)菌株Pi057C3和Pi050C3(也称为ATCC90586)使用MGISQ-2000RS测序仪生成基因组序列草案。因此,对于菌株Pi057C3,我们获得了包含14,134个重叠群的42.5-Mb组装基因组(164x覆盖),241的L50,45,748的N50,57.6%的CG含量,和12,147个ORF。对于菌株Pi050C3,我们收到了包含14,511个重叠群的43.3-Mb草图基因组(230x覆盖),L50为245,N50为45208,CG含量为57.7%,和12249个ORF。基因组序列已经以登录号JAKCXM000000000.1(菌株Pi057C3)和JAKCXL000000000.1(菌株Pi050C3)保藏在NCBI/DDBJ数据库中。
OBJECTIVE: Pythium insidiosum causes a difficult-to-treat infectious condition called pythiosis, with high morbidity and mortality. So far, genome data of at least 10 strains of P. insidiosum, primarily classified in the phylogenetic clades I and II, have been sequenced using various next-generation sequencing platforms. The MGI short-read platform was employed to obtain genome data of 2 clade-III strains of P. insidiosum (recently reclassified as Pythium periculosum) from patients in Thailand and the United States. This work is a part of our attempt to generate a comprehensive genome database from diverse pathogen strains.
METHODS: A 150-bp paired-end library was prepared from a gDNA sample of P. insidiosum (P. periculosum) strains Pi057C3 and Pi050C3 (also known as ATCC90586) to generate draft genome sequences using an MGISEQ-2000RS sequencer. As a result, for the strain Pi057C3, we obtained a 42.5-Mb assembled genome (164x coverage) comprising 14,134 contigs, L50 of 241, N50 of 45,748, 57.6% CG content, and 12,147 ORFs. For the strain Pi050C3, we received a 43.3-Mb draft genome (230x coverage) containing 14,511 contigs, L50 of 245, N50 of 45,208, 57.7% CG content, and 12,249 ORFs. The genome sequences have been deposited in the NCBI/DDBJ databases under the accession numbers JAKCXM000000000.1 (strain Pi057C3) and JAKCXL000000000.1 (strain Pi050C3).