■PyruscalleryanaDecne(玫瑰科),以其治疗特性而闻名,已知可以滋润肺部(去除干燥;缓解咳嗽),清热(作为解热药;febrifuge)和帮助解毒(缓解化脓性炎症;消除毒素)。然而,缺乏支持其在伤口愈合中功效的科学证据。
■这项研究调查了一年来收集的假棍样本,以探索代谢物的变化及其对皮肤伤口愈合活动的影响。
■P.从马祖群岛收集calleryana(PC)的树枝和叶子,台湾,跨越2018-2020年。提取物使用95%乙醇或水制备,我们评估了化学成分,总酚/三萜含量和抗氧化性能。通过LC-MS/MS和分子联网分析代谢物。通过MTT和迁移试验评估WS-1细胞的伤口愈合潜力,和基因表达分析,包括对照(DMSO)在内的测试,化合物1(3'-羟基苄基-4-羟基苯甲酸酯-4'-O-β-吡喃葡萄糖苷)和2(香草基花青素)(100µM),和阳性对照(抗坏血酸,100µM),持续24小时。
■根据使用的溶剂,观察到提取物组成的显着变化,在不同月份收集的提取物中具有不同的代谢组学特征。值得注意的是,化合物1和2对人真皮成纤维细胞没有细胞毒性作用,并且在100μM时显著加速伤口闭合。基因表达分析表明伤口愈合相关基因上调,包括MMP-1(基质金属蛋白酶-1)和COL1A1(胶原蛋白,类型1,阿尔法1)。
■这项研究报告了PC化合物有助于伤口愈合的第一个证据。利用全球天然产物社会分子网络(GNPS)和主成分分析(PCA)方法,我们公布了代谢组学概况,暗示了加速伤口愈合的潜力.
UNASSIGNED: Pyrus calleryana Decne (Rosaceae), renowned for its therapeutic properties, is known to moisturize the lungs (removing dryness; relieving cough), clear heat (acting as an antipyretic; febrifuge) and aid in detoxification (relieving pyogenic inflammation; eliminating toxins). However, scientific evidence supporting its efficacy in wound healing is lacking.
UNASSIGNED: This study investigated P. calleryana samples collected over a year to explore metabolite variations and their impact on skin wound-healing activities.
UNASSIGNED: P. calleryana (PC) twigs and leaves were collected from the Matsu Islands, Taiwan, spanning 2018-2020. Extracts were prepared using 95% ethanol or water, and we assessed the chemical composition, total phenolic/triterpenoid contents and antioxidant properties. Metabolites were analysed via LC-MS/MS and molecular networking. Wound healing potential was evaluated on WS-1 cells through MTT and migration assays, and gene expression analyses, with tests including control (DMSO), compounds 1 (3\'-hydroxylbenzyl-4-hydroxybenzoate-4\'-O-β-glucopyranoside) and 2 (vanilloylcalleryanin) (100 µM), and a positive control (ascorbic acid, 100 µM) for 24 h.
UNASSIGNED: Significant variations in extract compositions were observed based on the solvent used, with distinct metabolomic profiles in extracts collected during different months. Notably, compounds 1 and 2 showed no cytotoxic effects on human dermal fibroblast cells and significantly accelerated wound closure at 100 μM. A gene expression analysis indicated upregulation of wound healing-associated genes, including MMP-1 (matrix metalloproteinase-1) and COL1A1 (collagen, type 1, alpha 1).
UNASSIGNED: This study reports the first evidence of PC compounds aiding wound healing. Utilizing Global Natural Products Social Molecular Networking (GNPS) and principal component analysis (PCA) approaches, we unveiled metabolomic profiles, suggesting the potential to expedite wound-healing.