海洋碳氢化合物细菌可以使用多环芳烃作为碳和能源,这使得这些细菌对石油污染水域的生物修复非常有吸引力。然而,物种之间的基因组和代谢差异仍然是了解降解PAHs的进化和策略的研究主题。这项研究提出了红球菌MSA14,这是一种从下加利福尼亚州海洋沉积物中分离出的细菌,墨西哥,表现出对盐水环境的适应性,高水平的固有芘耐受性(>5gL-1),在27天内有效降解芘(0.2gL-1)30%。此外,通过使用萘和菲作为单独的碳源,该菌株具有多功能性。R.ruberMSA14的基因组测序揭示了一个跨越5.45Mbp的基因组,一个72kbp的质粒,和三个推定的巨质粒,长度在110和470Kbp之间。对R.ruberMSA14基因组的生物信息学分析揭示了56个基因,这些基因编码参与芳烃分解代谢的外周和中心途径的酶,烷烃,烯烃,和聚合物降解。在它的基因组中,R.ruberMSA14具有负责耐盐性和铁载体生产的基因。此外,R.ruberMSA14对13个参考基因组的基因组分析显示,所有比较的菌株都至少有一个基因参与烷烃和邻苯二酚降解途径。总的来说,生理测定和基因组分析表明,R.ruberMSA14是一种新的耐卤碱和碳氢化合物碎屑菌株,使其成为深入表征研究和生物修复过程的有希望的候选人,作为合成微生物联盟的一部分,以及更好地了解红球菌群的分解代谢潜力和功能多样性。
Marine hydrocarbonoclastic bacteria can use polycyclic aromatic hydrocarbons as carbon and energy sources, that makes these bacteria highly attractive for bioremediation in oil-polluted waters. However, genomic and metabolic differences between species are still the subject of study to understand the evolution and strategies to degrade PAHs. This study presents Rhodococcus ruber MSA14, an isolated bacterium from marine sediments in Baja California, Mexico, which exhibits adaptability to saline environments, a high level of intrinsic pyrene tolerance (> 5 g L- 1), and efficient degradation of pyrene (0.2 g L- 1) by 30% in 27 days. Additionally, this strain demonstrates versatility by using naphthalene and phenanthrene as individual carbon sources. The genome sequencing of R. ruber MSA14 revealed a genome spanning 5.45 Mbp, a plasmid of 72 kbp, and three putative megaplasmids, lengths between 110 and 470 Kbp. The bioinformatics analysis of the R. ruber MSA14 genome revealed 56 genes that encode enzymes involved in the peripheral and central pathways of aromatic hydrocarbon catabolism, alkane, alkene, and polymer degradation. Within its genome, R. ruber MSA14 possesses genes responsible for salt tolerance and siderophore production. In addition, the genomic analysis of R. ruber MSA14 against 13 reference genomes revealed that all compared strains have at least one gene involved in the alkanes and catechol degradation pathway. Overall, physiological assays and genomic analysis suggest that R. ruber MSA14 is a new haloalkalitolerant and hydrocarbonoclastic strain toward a wide range of hydrocarbons, making it a promising candidate for in-depth characterization studies and bioremediation processes as part of a synthetic microbial consortium, as well as having a better understanding of the catabolic potential and functional diversity among the Rhodococci group.