背景:证据表明衰老会影响牙髓的基本功能,比如防御能力和修复,因此影响保守牙髓治疗的成功。本研究旨在评估衰老对形态学的影响,迁移,扩散,和人牙髓细胞的免疫反应。
方法:用阿霉素处理细胞以诱导衰老,通过β-半乳糖苷酶染色证实。形态变化,通过扫描电子显微镜评估细胞增殖和迁移,锥虫蓝细胞,和划痕法,分别。通过使用PCR测定法测量促炎细胞因子肿瘤坏死因子α(TNF-α)和白介素6(IL-6)以及抗炎细胞因子转化生长因子β1(TGF-β1)和白介素10(IL-10)的基因来评估免疫反应的修饰。
结果:在衰老细胞中观察到细胞大小的增加和延伸数量的减少。在衰老细胞中也发现了增殖和迁移能力的降低。此外,炎性细胞因子TNF-α和IL-6的基因表达增加,IL-10和TGF-β1的基因表达减少,提示与免疫抑制相关的炎症情况加剧.
结论:细胞衰老可能是一种影响保守牙髓治疗预后的疾病,因为它会影响与这种治疗相关的原始细胞功能。
BACKGROUND: Evidence indicates that senescence can affect essential dental pulp functions, such as defense capacity and repair, consequently affecting the successes of conservative endodontic treatments. This study aims to evaluate the effects of senescence on the morphology, migration, proliferation, and immune response of human dental pulp cells.
METHODS: Cells were treated with doxorubicin to induce senescence, confirmed by β-galactosidase staining. Morphological changes, cellular proliferation, and migration were evaluated by scanning electron microscopy, trypan blue cells, and the scratch method, respectively. Modifications in the immune response were evaluated by measuring the genes for pro-inflammatory cytokines tumor necrosis factor alpha and interleukin (IL)-6 and anti-inflammatory cytokines transforming growth factor beta 1 and IL-10 using the real time polymerase chain reaction assay.
RESULTS: An increase in cell size and a decrease in the number of extensions were observed in senescent cells. A reduction in the proliferative and migratory capacity was also found in senescent cells. In addition, there was an increase in the gene expression of inflammatory cytokines tumor necrosis factor alpha and IL-6 and a decrease in the gene expression of IL-10 and transforming growth factor beta-1, suggesting an exacerbated inflammatory situation associated with immunosuppression.
CONCLUSIONS: Cellular senescence is possibly a condition that affects prognoses of conservative endodontic treatments, as it affects primordial cellular functions related to this treatment.