背景:肺顺应性,肺纤维化的生物标志物,通常是在全球范围内衡量的。超极化129Xe气体MRI提供了评估肺顺应性的潜力,允许可视化与纤维化相关的肺顺应性变化。
目的:使用超极化129Xe气体MRI评估肺纤维化大鼠模型的整体和区域肺顺应性。
方法:前瞻性。
■使用博来霉素诱导的纤维化模型(N=10)和盐水处理的对照(N=10)的20只Sprague-Dawley雄性大鼠。
■7-T,快速低角度拍摄(FLASH)序列。
结果:肺顺应性是通过迭代选择方法拟合来自分段129XeMRI的肺体积来确定的,相应的气道压力。同样,通过计算机断层扫描获得肺顺应性以进行交叉验证.肺顺应性的方向依赖性由不同方向的区域肺顺应性比(R)表征。肺功能试验(PFTs)和组织学分析用于验证肺纤维化模型并评估其与129Xe肺顺应性的相关性。
方法:夏皮罗-威尔克试验,不成对和成对t检验,Mann-WhitneyU和Wilcoxon符号秩检验,和皮尔逊相关系数。P<0.05被认为具有统计学意义。
结果:对于整个肺,用129Xe气体MRI测量的全球和区域肺顺应性显示两组之间存在显着差异,并与使用PFTs测量的整体肺顺应性相关(全局:r=0.891;区域:r=0.873)。此外,对于对照组,地区之间的平均地区遵守情况存在显著差异,例如,心尖和基底肺为0.37(0.32,0.39)×10-4mL/cmH2O和0.47(0.41,0.56)×10-4mL/cmH2O,分别。纤维化组和对照组的根尖-基底方向R分别为1.12±0.09和1.35±0.13,分别,表明存在显著差异。
结论:我们的研究结果证明了使用超极化气体MRI评估局部肺顺应性的可行性。
方法:2技术效果:第一阶段。
BACKGROUND: Lung compliance, a biomarker of pulmonary fibrosis, is generally measured globally. Hyperpolarized 129Xe gas MRI offers the potential to evaluate lung compliance regionally, allowing for visualization of changes in lung compliance associated with fibrosis.
OBJECTIVE: To assess global and regional lung compliance in a rat model of pulmonary fibrosis using hyperpolarized 129Xe gas MRI.
METHODS: Prospective.
UNASSIGNED: Twenty Sprague-Dawley male rats with bleomycin-induced fibrosis model (N = 10) and saline-treated controls (N = 10).
UNASSIGNED: 7-T, fast low-angle shot (FLASH) sequence.
RESULTS: Lung compliance was determined by fitting lung volumes derived from segmented 129Xe MRI with an iterative selection method, to corresponding airway pressures. Similarly, lung compliance was obtained with computed tomography for cross-validation. Direction-dependencies of lung compliance were characterized by regional lung compliance ratios (R) in different directions. Pulmonary function tests (PFTs) and histological analysis were used to validate the pulmonary fibrosis model and assess its correlation with 129Xe lung compliance.
METHODS: Shapiro-Wilk tests, unpaired and paired t-tests, Mann-Whitney U and Wilcoxon signed-rank tests, and Pearson correlation coefficients. P < 0.05 was considered statistically significant.
RESULTS: For the entire lung, the global and regional lung compliance measured with 129Xe gas MRI showed significant differences between the groups, and correlated with the global lung compliance measured using PFTs (global: r = 0.891; regional: r = 0.873). Additionally, for the control group, significant difference was found in mean regional compliance between areas, eg, 0.37 (0.32, 0.39) × 10-4 mL/cm H2O and 0.47 (0.41, 0.56) × 10-4 mL/cm H2O for apical and basal lung, respectively. The apical-basal direction R was 1.12 ± 0.09 and 1.35 ± 0.13 for fibrosis and control groups, respectively, indicating a significant difference.
CONCLUSIONS: Our findings demonstrate the feasibility of using hyperpolarized gas MRI to assess regional lung compliance.
METHODS: 2 TECHNICAL EFFICACY: Stage 1.