葡萄(VitisviniferaL.)是新鲜消费和葡萄酒生产的重要作物。葡萄园受到几种经济上重要的细菌和真菌疾病的攻击,需要定期进行农药处理。其中,丁香假单胞菌pv。丁香(PS。丁香)和灰葡萄孢(B.cinerea)感染造成巨大的经济损失。新鲜水果市场已转向功能性天然食品,具有明显的健康益处,并减少了生产链中化学品的使用。乳酸菌(LAB)具有生物防腐作用,可根据消费者的需求应用于确保食品安全。在本研究中,使用对Ps具有潜在抗菌作用的微生物的可能性。研究了丁香和灰霉病在食用葡萄生产中的应用。Lactiplantibacillus属的LAB可以通过释放乳酸成为病原菌和真菌的天然拮抗剂,乙酸,乙醇,培养基中的二氧化碳和细菌素。本研究的重点是9种植物乳杆菌(Lp。植物区系)从自发发酵的酸味中分离出的菌株。物种特异性PCR鉴定了用于部分recA基因扩增的分离的LAB,其扩增子大小为318bp。RAPD-PCR分析显示单个菌株的种内多样性。13种植物霉素样肽(PlnA,PlnB,PlnC,PlnD,PlnEF,PlnG,PlnI,PlnJ,PlnK,PlnN,PlnNC8,PlnS,和PlnW)由分离的Lp产生。用基因特异性引物进行PCR检测。未来工业应用的关键特征是它们的抗菌性能。培养基和无细胞上清液(CFS)用于建立Lp的体外抗菌活性。抗Ps的植物区系。丁香和B.cinerea,并观察到对植物病原体发育的抑制作用。通过在体内实验中用这些病原体感染食用葡萄来评估所有菌株的CFS(无细胞上清液)的抑制作用。Lp.植物区系Q4在体外和体内表现出对病原体的最有效抑制,这表明了它作为生物防治剂的潜在用途,对抗葡萄上的浆果腐烂和灰腐病,由Ps引起。丁香和B。
Grapes (Vitis vinifera L.) are an essential crop for fresh consumption and wine production. Vineyards are attacked by several economically important bacterial and fungal diseases that require regular pesticide treatment. Among them, Pseudomonas syringae pv. syringae (Ps. syringae) and Botrytis cinerea (B. cinerea) infections cause huge economic losses. The fresh fruit market has shifted to functional natural foodstuffs with clear health benefits and a reduced use of chemicals along the production chain. Lactic acid bacteria (LAB) have a biopreservative effect and are applied to ensure food safety in response to consumers\' demands. In the present study, the possibilities of using microorganisms with a potential antimicrobial effect against Ps. syringae and B. cinerea in the production of table grapes were investigated. LAB of the genus Lactiplantibacillus can be a natural antagonist of pathogenic bacteria and fungi by releasing lactic acid, acetic acid, ethanol, carbon dioxide and bacteriocins in the medium. The present study focuses on the characterization of nine Lactiplantibacillus plantarum (Lp. plantarum) strains isolated from spontaneously fermented sourdoughs. Species-specific PCR identified the isolated LAB for partial recA gene amplification with an amplicon size of 318 bp. RAPD-PCR analysis showed the intraspecific diversity of the individual strains. Thirteen plantaricin-like peptides (PlnA, PlnB, PlnC, PlnD, PlnEF, PlnG, PlnI, PlnJ, PlnK, PlnN, PlnNC8, PlnS, and PlnW) produced by isolated Lp. plantarum strains were detected by PCR with gene-specific primers. The key features for future industrial applications were their antimicrobial properties. The culture medium and cell-free supernatant (CFS) were used to establish in vitro antimicrobial activities of Lp. plantarum strains against Ps. syringae and B. cinerea, and inhibition of phytopathogen development was observed. The inhibitory effect of the CFS (cell-free supernatant) of all strains was assessed by infecting table grapes with these pathogens in in vivo experiments. Lp. plantarum Q4 showed the most effective suppression of the pathogens both in vitro and in vivo, which indicates its potential use as a biocontrol agent against berry rot and grey rot on grapes, caused by Ps. syringae and B. cinerea.