细胞因子信号转导抑制因子(SOCS)基因家族是一组参与细胞因子信号转导负调控的基因。该家族的成员在调节免疫和炎症过程中起着至关重要的作用。然而,尚未在具有经济意义的大黄鱼(Larimichthyscrocea)中对这些基因进行全面研究。在这项研究中,共有13个SOCS基因(LcSOCS1a,LcSOCS1b,LcSOCS2,LcSOCS3a,LcSOCS3b,LcSOCS4,LcSOCS5a,LcSOCS5b,LcSOCS6,LcSOCS7a,LcSOCS7b,LcCISHa和LcCISHb)在大黄乳杆菌中进行了鉴定和分析。系统发育树揭示了SOCS基因在进化中的高度保守性,基因结构和基序分析表明同一亚家族中LcSOCSs的结构高度相似。此外,LcSOCSs的表达模式显示,LcSOCS1b在急性低氧胁迫下,但它在斑驳病菌感染后的整个过程中显著上调,揭示了它对两种压力的不同免疫效应。此外,LcSOCS2a,LcSOCS6和LcSOCS7a仅参与急性低氧应激,而LcSOCS5a对P.plecoglossicida感染更敏感。总之,这些结果表明,SOCS基因参与了对生物和非生物刺激的应激反应,为深入研究SOCS基因功能奠定基础。
The suppressor of cytokine signaling (SOCS) gene family is a group of genes involved in the negative regulation of cytokine signal transduction. The members of this family play a crucial role in regulating immune and inflammatory processes. However, comprehensive investigations of these genes have not yet been conducted in the economically significant fish large yellow croaker (Larimichthys crocea). In this study, a total of 13 SOCS genes (LcSOCS1a, LcSOCS1b, LcSOCS2, LcSOCS3a, LcSOCS3b, LcSOCS4, LcSOCS5a, LcSOCS5b, LcSOCS6, LcSOCS7a, LcSOCS7b, LcCISHa and LcCISHb) were identified and analyzed in L. crocea. The phylogenetic tree revealed a high conservation of SOCS genes in evolution, and the gene structure and motif analysis indicated a high similarity in the structure of LcSOCSs in the same subfamily. In addition, the expression patterns of LcSOCSs showed that LcSOCS1b was significantly down-regulated in all time under acute hypoxia stress, but it was markedly up-regulated throughout the entire process after P. plecoglossicida infection, revealing its different immune effects to two stresses. Besides, LcSOCS2a, LcSOCS6 and LcSOCS7a only participated in acute hypoxic stress, while LcSOCS5a was more sensitive to P. plecoglossicida infection. In summary, these results indicated that SOCS genes were involved in stress responses to both biological and non-biological stimuli, setting the foundation for deeper study on the functions of SOCS genes.