Prunusconradinae(Cerasus亚属,玫瑰科)是中国野生樱花的重要种质资源。为确保本研究的全面性,我们使用了一个大的样本量(12个种群,包括244个个体),其中涉及东部的P.conradinae的新鲜叶子,中央,和中国西南部。我们结合形态学和分子证据(三个叶绿体DNA(cpDNA)序列和一个核DNA(nrDNA)序列)来检查P的种群变异和分化。我们的结果显示,中央,东,西南地区是保证未来有足够种质资源的重要地区。我们还发现了对新变体的支持,P.Conradinaevar。rubrum.我们观察到P.conradinae内的高遗传多样性(单倍型多样性[Hd]=0.830;核糖型多样性[Rd]=0.798),具有新颖的遗传变异和种群间独特的家谱结构。种群之间存在遗传变异,种群和三个地理群体之间存在系统地理结构(中部,东,和中国西南地区)。西南地区遗传分化系数最低,基因交换明显,而华中地区分化明显。在这三个地理群体中,我们确定了两个不同的血统:华东血统(华中和华东)和西南血统((华中和西南)和华东)。由于地理隔离,这两个谱系起源于大约438万年前(Mya)的上新世早期。在上新世,以3.32Mya(95%HPD:1.12-5.17Mya)从中部扩展到东部。从华东地区到西南地区,分化时间为2.17Mya(95%(HPD:0.47-4.54Mya),这表明,中国中部和东部的P。在第四纪更新世早期,P.conradinae的种群在1.10Mya(95%HPD:0.11-2.85Mya)左右经历了从中部到西南地区的分化。华东地区的东南地区,在武夷山附近,可能是P.conradinae的避难所。本研究为分类奠定了理论基础,identification,养护,油菜种质资源的开发。
Prunus conradinae (subgenus Cerasus, Rosaceae) is a significant germplasm resource of wild cherry blossom in China. To ensure the comprehensiveness of this study, we used a large sample size (12 populations comprising 244 individuals) which involved the fresh leaves of P. conradinae in Eastern, Central, and Southwestern China. We combined morphological and molecular evidence (three chloroplast DNA (cpDNA) sequences and one nuclear DNA (nr DNA) sequence) to examine the population of P. conradinae variation and differentiation. Our results revealed that Central, East, and Southwest China are important regions for the conservation of P. conradinae to ensure adequate germplasm resources in the future. We also found support for a new variant, P. conradinae var. rubrum. We observed high genetic diversity within P. conradinae (haplotype diversity [Hd] = 0.830; ribotype diversity [Rd] = 0.798), with novel genetic variation and a distinct genealogical structure among populations. There was genetic variation among populations and phylogeographic structure among populations and three geographical groups (Central, East, and Southwest China). The genetic differentiation coefficient was the lowest in the Southwest region and the gene exchange was obvious, while the differentiation was obvious in Central China. In the three geographic groups, we identified two distinct lineages: an East China lineage (Central China and East China) and a Southwest China lineage ((Central China and Southwest China) and East China). These two lineages originated approximately 4.38 million years ago (Mya) in the early Pliocene due to geographic isolation. P. conradinae expanded from Central China to East China at 3.32 Mya (95% HPD: 1.12-5.17 Mya) in the Pliocene. The population of P. conradinae spread from East China to Southwest China, and the differentiation time was 2.17 Mya (95% (HPD: 0.47-4.54 Mya), suggesting that the population of P. conradinae differentiated first in Central and East China. The population of P. conradinae experienced differentiation from Central China to Southwest China around 1.10 Mya (95% HPD: 0.11-2.85 Mya) during the early Pleistocene of the Quaternary period. The southeastern region of East China, near Mount Wuyi, likely serves as a refuge for P. conradinae. This study establishes a theoretical foundation for the classification, identification, conservation, and exploitation of germplasm resources of P. conradinae.