动脉粥样硬化是冠状动脉疾病的初步病因,致死人数最多的疾病之一。体力活动是抑制动脉粥样硬化恶化的有效策略。有证据表明,蛋白质组谱的变化与动脉粥样硬化的发展高度相关,但运动改善动脉粥样硬化的机制尚未从蛋白质组学的角度进行研究。因此,蛋白质组学谱可以进一步阐明运动干预对ApoE基因敲除动脉粥样硬化模型和高脂饮食干预的系统影响。在目前的研究中,Apoeem1Narl/Narl小鼠随机分配到正常饮食(ND),西方饮食(WD),和WD进行12周运动干预(WDEX)组。WD和WDEX组之间的血浆蛋白质组显示出显着差异,和十大主要途径,包括心血管疾病(CVD)-血液病,炎症性疾病,传染病,炎症反应,细胞间信号和相互作用,结缔组织疾病_炎性疾病,代谢性疾病_机体损伤和异常,细胞间信号和相互作用,结缔组织疾病_炎性疾病,和内分泌系统疾病_胃肠道疾病,等。,是通过IPA分析产生的。15种蛋白质(MYOCD,PROS1,C2,SERPINA10,CRP,F5,C5,CFB,FGG,CFH,F12,PRDX2,PROZ,PPIA,和HABP2)严重参与CVD-血液病途径的WD和WDEX组之间存在显着差异。在目前的研究中,运动可以明显减轻WD组引起的C5和炎症反应,并改善动脉粥样硬化。因此,运动可以通过调节C5水平和先天免疫来减轻趋化性,从而缓解西方饮食诱导的肥胖小鼠动脉粥样硬化的发病机理。
Atherosclerosis is the preliminary cause of coronary artery disease, one of the diseases that account for the largest number of fatal mortalities. Physical activity is an effective strategy to restrain atherosclerosis from deterioration. Evidence indicated that changes in the proteomic profile are highly associated with atherosclerosis development, but the mechanism behind exercise for atherosclerosis amelioration has not yet been investigated from a proteomics perspective. Hence, the proteomic profiles could further elucidate the systematic effects of exercise intervention on ApoE knockout atherosclerotic model and high-fat-diet intervention. In the current study, Apoeem1Narl/Narl mice were randomly allocated into a normal diet (ND), Western diet (WD), and WD with 12-week exercise intervention (WD EX) groups. The plasma proteome between WD and WD EX groups demonstrate the significant difference, and ten major pathways, including cardiovascular disease (CVD)-hematological disease, inflammatory disease, infectious diseases, inflammatory response, cell-to-cell signaling and interaction, connective tissue disorders_inflammatory disease, metabolic disease_organismal injury and abnormalities, cell-to-cell signaling and interaction, connective tissue disorders_inflammatory disease, and endocrine system disorders_gastrointestinal disease, etc., were generated by the IPA analysis. The 15 proteins (MYOCD, PROS1, C2, SERPINA10, CRP, F5, C5, CFB, FGG, CFH, F12, PRDX2, PROZ, PPIA, and HABP2) critically involved in CVD-hematological disease pathway showed significant difference between WD and WD EX groups. In current study, exercise could significantly alleviate the significantly elevated C5 and inflammation induced by the WD group in accordance with amelioration of atherosclerosis. Therefore, exercise could mitigate chemotaxis through the modulation of the C5 level and innate immunity, thereby alleviating the pathogenesis of atherosclerosis in Western-diet-induced obese mice.