姜黄根茎(姜黄)和黑孜然种子(Nigellasativa)是用于治疗尼日利亚民族医学中的癌症和其他慢性炎症性疾病的多草药成分。以前的研究表明,抗氧化剂,抗炎,和个体植物提取物的抗癌活性。然而,这两种香料的结合形式并没有在生物学上得到强化。因此,这项研究从组合香料中获得精油(EOs),并评估其对自由基的抑制作用,蛋白质变性,和癌症扩散。通过加氢蒸馏(HD)提取EO,并通过气相色谱-质谱(GC-MS)进行表征。基于DPPH进行体外抗氧化评估,过氧化氢(H2O2),一氧化氮(NO),和铁离子(Fe3+)自由基清除试验。在MTT细胞活力测定后,测定了油对非致瘤细胞系(HEK293)和癌性细胞系(HepG2和HeLa)的细胞毒性。还进行了油成分的硅分子对接分析。提供了六批EOsI-VI,由22个主要成员组成,芳香的Ar-姜黄酮是最突出的化合物。在重复HD和作为组合时,油的生物活性有显著改善。批次VI油表现出最好的活性,对HepG2细胞系的细胞毒性(CC50)为10.16±1.69µg/100µL,与5-氟尿嘧啶(标准,CC50=8.59±1.33µg/100µL)。在硅分子对接中建议δ-姜黄烯,Ar-姜黄烯,Ar-turmerol,和Ar-姜黄酮在有希望的化合物中,基于它们与NOX2,NF-κB的高结合能分数,和mdm2蛋白质。总之,来自姜黄黑孜然的油组合具有相当大的抑制自由基的能力,蛋白质变性,和癌症扩散。这项研究的发现进一步强调了姜黄黑孜然作为多草药成分的有效性。
Turmeric rhizomes (Curcuma longa) and black cumin seeds (Nigella sativa) are polyherbal ingredients used for the management of cancer and other chronic inflammatory diseases in Nigerian ethnomedicine. Previous studies have shown the antioxidant, anti-inflammatory, and anticancer activities of the individual plant extracts. However, the two spices have not been biologically potentiated in their combined form. Therefore, this study obtained essential oils (EOs) from the combined spices and evaluated their inhibitory effects on free radicals, protein denaturation, and cancer proliferation. The EOs were extracted by hydro-distillation (HD) and characterized by gas chromatography-mass spectrometry (GC-MS). In vitro antioxidant assessment was conducted based on DPPH, hydrogen peroxide (H2O2), nitric oxide (NO), and ferric ion (Fe3+) radical scavenging assays. The cytotoxicity of the oil against non-tumorigenic (HEK293) and cancerous (HepG2 and HeLa) cell lines was determined following the MTT cell viability assay. An in silico molecular docking analysis of the oil constituents was also performed. Six batches of EOs I-VI were afforded, comprising twenty-two major constituents, with aromatic Ar-turmerone being the most prominent compound. There was a marked improvement in the bioactivity of the oils upon repeated HD and as a combination. The batch VI oil exhibited the best activity, with a cytotoxicity (CC50) of 10.16 ± 1.69 µg/100 µL against the HepG2 cell line, which was comparable to 5-fluorouracil (standard, CC50 = 8.59 ± 1.33 µg/100 µL). In silico molecular docking suggested δ-curcumene, Ar-curcumene, Ar-turmerol, and Ar-turmerone among the promising compounds based on their high binding energy scores with NOX2, NF-κB, and mdm2 proteins. In conclusion, the oils from the turmeric-black cumin combined possess a considerable inhibition ability against free radicals, protein denaturation, and cancer proliferation. This study\'s findings further underscore the effectiveness of turmeric-black cumin as a polyherbal medicinal ingredient.