■程序性死亡配体1(PD-L1)评分是预测免疫疗法反应的重要伴随诊断。免疫组织化学可以准确评估常规石蜡包埋组织中PD-L1的表达。然而,脱钙或脱色素组织是否仍然准确,可以作为伴随诊断存在争议。本研究试图通过分析不同时间脱钙和脱色对PD-L1表达的影响来解决这一争议。
■选择胎盘组织进行组织微阵列,根据6、12、24、36和48小时的时间梯度进行脱钙,根据1、5、15、30和60分钟的时间梯度进行脱色。观察并定量不同时间点的PD-L1表达强度。选择10例PD-L1阳性食管鳞癌标本进行脱钙治疗,还有PD-L1.综合积极评分(CPS),比较脱钙前后肿瘤比例评分(TPS)和免疫细胞比例评分(IPS)及阳性率。
■胎盘脱钙后,PD-L1阳性强度减弱,平均光密度(AOD)值随脱钙时间的延长而降低,24h时与对照组相比显著降低(P<0.05),36、48h与对照组比较差异有统计学意义(P<0.01)。高锰酸钾脱色后,PD-L1阳性强度明显减弱。此外,脱色时间达到5min后AOD值较对照组明显降低(P<0.01)。10例PD-L1阳性食管鳞癌24h脱钙治疗,虽然PD-L1评分有一定程度的下降(P>0.05),阳性率可达90%。治疗36小时后,PD-L1评分下降,CPS和IPS评分显著降低(P<0.05),阳性率仅为50%。
■高锰酸钾脱色显著降低PD-L1表达,即使在更短的时间内,影响结果的准确性。PD-L1在24h脱钙内保持较高的准确性。以上结果对临床选择免疫治疗具有一定的参考价值。
UNASSIGNED: Programmed death ligand 1 (PD-L1) score is an important companion diagnosis to predict the response to immunotherapy. Immunohistochemistry can accurately assess the expression of PD-L1 in routine paraffin-embedded tissue. However, whether decalcified or depigmented tissue is still accurate and can be used as a companion diagnosis is controversial. This study attempts to resolve this controversy by analyzing the effects of decalcification and depigmentation at different times on PD-L1 expression.
UNASSIGNED: Placental tissues were selected for tissue microarray, decalcification was performed according to time gradients of 6, 12, 24, 36, and 48 h, and depigmentation was performed according to time gradients of 1, 5, 15, 30, and 60 min. The intensity of PD-L1 expression at different time points was observed and quantified. Ten PD-L1-positive esophageal squamous carcinoma samples were selected for decalcification treatment, and the PD-L1. Combined Positive Score (CPS), Tumor Proportion Score (TPS) and Immunocyte Proportion Score (IPS) and the positivity rates were compared before and after decalcification.
UNASSIGNED: After the placenta was decalcified, the intensity of PD-L1 positivity diminished, and the average optical density (AOD) value decreased with the prolongation of decalcification time and decreased significantly (P<0.05) at 24 h compared with the control group, and significantly (P<0.01) at 36 and 48 h compared with the control group. The intensity of PD-L1 positivity was weakened considerably after the treatment with potassium permanganate depigmentation. In addition, the AOD value decreased significantly (P<0.01) after the depigmentation time reached 5 min compared with the control group. Ten cases of PD-L1 positive esophageal squamous carcinoma were treated with 24 h decalcification, although the PD-L1 score decreased to a certain degree (P>0.05), and the positivity rate could reach 90%. After 36 h treatment, PD-L1 scores decreased, the CPS and IPS scores decreased significantly (P<0.05), and the positive rate was only 50%.
UNASSIGNED: Potassium permanganate depigmentation significantly reduces PD-L1 expression, even for a shorter time, affecting the accuracy of the results. The accuracy of PD-L1 remained high within 24 h decalcification. The above results have certain reference value for clinical selection of immunotherapy.