背景:胃癌是胃肠道最常见的恶性肿瘤之一,这对人类健康有很大的负面影响。
目的:CCL趋化因子在多种肿瘤微环境中发挥重要作用;令人惊讶的是,胃癌与CCL趋化因子的关联有限.
方法:在我们的研究中,我们综合利用了生物信息学分析工具和数据库,如cBioPortal,UALCAN,GEPIA,遗传狂躁症,STRING,和TRRUST阐明CCL趋化因子在胃癌中的临床意义和生物学功能。
结果:CCL1/3/4/5/7/8/14/15/18/20/21/22/26mRNA表达上调,而CCL2/11/13/16/17/19/23/24/25/28的mRNA表达水平下调。与胃癌病理分期显著相干的趋化因子为CCL2/11/19/21。在胃癌中,CCL趋化因子的表达水平与无病生存率无关,但CCL14的低表达与较长的总生存期显著相关.其中,与CCL趋化因子调节相关的只有10个转录因子(RELA,NFKB1,STAT6,IRF3,REL,SPI1、STAT1、STAT3、JUN和SP1)。CCL趋化因子的主要生物学过程和功能富集是诱导细胞定向迁移。
结论:这些结果可能表明CCL趋化因子可能是胃癌的免疫治疗靶标和有希望的预后生物标志物。
BACKGROUND: Gastric cancer is one of the most common malignant tumours of the gastrointestinal tract, which has a significant negative impact on human health.
OBJECTIVE: CCL chemokines play important roles in a variety of tumor microenvironments; nevertheless, gastric cancer has surprisingly limited associations with CCL chemokines.
METHODS: In our study, we comprehensively utilized bioinformatics analysis tools and databases such as cBioPortal, UALCAN, GEPIA, GeneMANIA, STRING, and TRRUST to clarify the clinical significance and biology function of CCL chemokines in gastric cancer.
RESULTS: The mRNA expression levels of CCL1/3/4/5/7/8/14/15/18/20/21/22/26 were up-regulated, while the mRNA expression levels of CCL2/11/13/16/17/19/23/24/25/28 were down-regulated. The chemokine significantly associated with the pathological stage of gastric cancer is CCL2/11/19/21. In gastric cancer, the expression level of CCL chemokines was not associated with disease-free survival, but low expression of CCL14 was significantly associated with longer overall survival. Therein, associated with the regulation of CCL chemokines are only 10 transcription factors (RELA, NFKB1, STAT6, IRF3, REL, SPI1, STAT1, STAT3, JUN and SP1). The major biological process and functional enrichment of CCL chemokines are to induce cell-directed migration.
CONCLUSIONS: These results may indicate that CCL chemokines may be immunotherapeutic targets and promising prognostic biomarkers for gastric cancer.