发现母乳中的新兴污染物将有助于了解母乳对人体健康的危害。然而,很难在复杂样品中存在的数千种化合物中识别关键化合物。在这项研究中,开发了一种筛选具有生物富集潜力的化合物的方法。该方法可以减少需要结构鉴定的化合物的数量,因为生物蓄积性化合物的分配特性可以通过其保留行为映射到GC×GC色谱上。分析了来自中国七个省的20个样本。从3,000多种化合物中选择了1,286种具有生物积累潜力的化合物。六十二种化合物,包括芳香化合物,邻苯二甲酸酯,和酚类等.,以高置信度识别,然后量化。其中,在母乳中首次发现了27种化合物。发现三种邻苯二甲酸酯增塑剂和两种酚类抗氧化剂的浓度明显高于其他化合物。毒理学优先指数方法被应用于优先考虑其浓度的化合物,检测频率和八种毒性作用。优先化表明13种化合物,包括邻苯二甲酸二(2-乙基己基)酯,邻苯二甲酸二丁酯,1,3-二叔丁基苯,菲,2,6-二叔丁基-1,4-苯醌,2,4-二叔丁基苯酚,和其他人,显示出更高的健康风险。同时,一些具有特定毒性作用的高风险的化合物,如苯并噻唑和香叶基丙酮,仍然值得注意。这项研究对于评估人类接触有机化合物的风险非常重要。
Discovery of emerging pollutants in breast milk will be helpful for understanding the hazards to human health. However, it is difficult to identify key compounds among thousands present in complex samples. In this study, a method for screening compounds with bioaccumulation potential was developed. The method can decrease the number of compounds needing structural identification because the partitioning properties of bioaccumulative compounds can be mapped onto GC×GC chromatograms through their retention behaviors. Twenty pooled samples from seven provinces in China were analyzed. 1,286 compounds with bioaccumulation potential were selected from over 3,000 compounds. Sixty-two compounds, including aromatic compounds, phthalates, and phenolics etc., were identified with a high level of confidence and then quantified. Among them, twenty-seven compounds were found for the first time in breast milk. Three phthalate plasticizers and two phenolic antioxidants were found in significantly higher concentrations than other compounds. A toxicological priority index approach was applied to prioritize the compounds considering their concentrations, detection frequencies and eight toxic effects. The
prioritization indicated that 13 compounds, including bis(2-ethylhexyl) phthalate, dibutyl phthalate, 1,3-di-tert-butylbenzene, phenanthrene, 2,6-di-tert-butyl-1,4-benzoquinone, 2,4-di-tert-butylphenol, and others, showed higher health risks. Meanwhile, some compounds with high risk for a particular toxic effect, such as benzothiazole and geranylacetone, were still noteworthy. This study is important for assessing the risks of human exposure to organic compounds.