目的:我们研究的目的是评估储存温度和分析时间对动脉血气参数的影响,以扩展CLSI建议。
方法:12个参数的稳定性(pH,二氧化碳,二氧化碳,Na+,K+,Ca2+,葡萄糖,乳酸,血红蛋白,氧合血红蛋白,碳氧血红蛋白,在室温和+4°C下研究了通过GEMPREMIER™5000血气分析仪测量的高铁血红蛋白)(52名患者)。储存时间为30、45、60、90和120分钟。根据与基线的差异评估稳定性,与应用于基线值的分析物特定测量不确定性的差异,以及变异对临床解释的影响。
结果:在室温下,除乳酸盐外的所有参数都保持稳定至少60分钟。在没有修改临床解释的情况下,在T45和T60的pH和在T60的pCO2观察到统计学上的显着差异。对于乳酸,对T45的临床解释进行了修改,数值超出了测量不确定度定义的可接受范围.除pO2外的所有参数在+4℃下保持稳定至少120分钟。
结论:室温下1小时的运输与除乳酸以外的所有研究分析的性能相容。如果延迟超过30分钟,样品应放置在+4°C进行乳酸测量。如果样品储存在冰上,重要的是要注意pO2不能被解释。
The objective of our study is to evaluate the effect of storage temperature and time to analysis on arterial blood gas parameters in order to extend the CLSI recommendations.
Stability of 12 parameters (pH, pCO₂, pO₂, Na+, K+, Ca2+, glucose, lactate, hemoglobin, oxyhemoglobin, carboxyhemoglobin, methemoglobin) measured by GEM PREMIER™ 5000 blood gas analyzer was studied at room temperature and at +4 °C (52 patients). The storage times were 30, 45, 60, 90 and 120 min. Stability was evaluated on the difference from baseline, the difference from the analyte-specific measurement uncertainty applied to the baseline value, and the impact of the variation on the clinical interpretation.
At room temperature, all parameters except the lactate remained stable for at least 60 min. A statistically significant difference was observed for pH at T45 and T60 and for pCO2 at T60 without modification of clinical interpretation. For lactate, clinical interpretation was modified from T45 and values were outside the range of acceptability defined by the measurement uncertainty. All parameters except pO2 remained stable for at least 120 min at +4 °C.
A one-hour transport at room temperature is compatible with the performance of all the analyses studied except lactate. If the delay exceeds 30 min, the sample should be placed at +4 °C for lactate measurement. If the samples are stored in ice, it is important to note that the pO2 cannot be interpreted.