目的:宠物猪是医学上越来越感兴趣的物种,和基于证据的输血实践是必要的。本研究的目的是量化3种血型(Bg)表型(“A”和“Aweak”由EAAAA和EAAA0产生,\"0\"来自EAA00,或来自EAA00或SSS等位基因的\“-”)在宠物猪中,并使用人类血型卡(EldonCard)比较结果,标准盐水凝集(SSA),和聚合酶链反应(PCR)测序。
方法:横断面研究。
方法:大学兽医教学医院。
方法:来自路易斯安那州的97头宠物猪。
方法:从随机选择的不同品种的宠物猪中抽取血液,用EDTA抗凝,并根据制造商的指示或标准实验室技术使用每个调查测试进行打字。将用于PCR分析的样品储存在-80°C直至分析。通过EldonCard筛选表型。用卡方检验研究Bg与性别之间的关系,在P<0.05时具有显著性。使用Kappa(κ)统计量来测量3个测试之间的一致性水平。
结果:腹猪占该种群的大多数(84.5%),男性52人(53.6%),女性45人(46.4%)。基因型频率为30%,30%,和40%的“EAAAA,\"\"EAAA0,\"和\"EAA00,\"分别。根据EldonCard,38种表型被归类为“A,“5为”虚弱,\"和54为\"0\"或\"-。“Bg的结果相同,在90%(45/50)的样品中使用3种测试技术。EldonCard和PCR之间的协议几乎是完美的(49/50[98%],κ=0.959;P<0.001)。SSA和PCR之间的协议,EldonCard和SSA相当大(46/50[92%],κ=0.803,P<0.001和93/97[95.9%],κ=0.764,P<0.001)。
结论:最常见的血型是“0”或“-”(55.7%),其次是“A”(39.2%)和“Aweak”(5.1%)。EldonCard和PCR测试之间有很强的一致性。EldonCard允许快速可靠的表型鉴定(“A,\"\"虚弱,\"and\"0\"or\"-\")andrepresentsaclinicalapplicablelaboratorymethodforbloodtypinginpetpig.
OBJECTIVE: Pet pigs are a species of growing medical interest, and evidence-based practices for blood transfusions are needed. The objectives of this study were to quantify the prevalence of 3 blood group (Bg) phenotypes (\"A\" and \"Aweak \" resulting from EAAAA and EAAAO , \"0\" from EAA00 , or \"-\" from EAA00 or SSS alleles) in pet pigs and compare results using a human blood-typing card (EldonCard), standard saline agglutination (SSA), and polymerase chain reaction (PCR) sequencing.
METHODS: Cross-sectional study.
METHODS: University veterinary teaching hospital.
METHODS: Ninety-seven pet pigs from Louisiana.
METHODS: Blood was sampled from randomly selected pet pigs of various breeds, anticoagulated with EDTA, and typed using each investigated test according to the manufacturers\' directions or standard laboratory technique. Samples for PCR analysis were stored at -80°C until analysis. Phenotypes were screened via EldonCard. Association between Bg and sex was investigated using chi-square test, with significance at P < 0.05. Kappa (κ) statistic was used to measure the level of agreement between the 3 tests.
RESULTS: Pot-bellied pigs represented the majority (84.5%) of this population, with 52 (53.6%) males and 45 (46.4%) females. Genotypic frequencies were 30%, 30%, and 40% for \"EAAAA ,\" \"EAAAO ,\" and \"EAA00 ,\" respectively. Based on EldonCard, 38 phenotypes were classified as \"A,\" 5 as \"Aweak ,\" and 54 as \"0\" or \"-.\" Results were identical for Bg, with the 3 tested techniques in 90% (45/50) of samples. Agreement between EldonCard and PCR was almost perfect (49/50 [98%], κ = 0.959; P < 0.001). Agreement between SSA and PCR, and EldonCard and SSA was substantial (46/50 [92%], κ = 0.803, P < 0.001 and 93/97 [95.9%], κ = 0.764, P < 0.001, respectively).
CONCLUSIONS: The most common blood type was \"0\" or \"-\" (55.7%), followed by \"A\" (39.2%) and \"Aweak \" (5.1%). There was strong agreement between EldonCard and PCR testing. EldonCard allowed for rapid and reliable phenotype identification (\"A,\" \"Aweak ,\" and \"0\" or \"-\") and represents a clinically applicable laboratory method for blood typing in pet pigs.