目的:肌酸激酶(CK)和醛缩酶是传统上用于研究肌肉损伤(MD)的标志物。由于CK测定对肌肉损伤更有特异性,在常规实验室测试中对这两种测定的需求将需要额外的成本。
方法:在2019-2020年间进行的回顾性观察性研究。研究了218例患者的CK和醛缩酶浓度。分析CK和醛缩酶的ROC曲线以检测肌肉损伤。为两种策略选择截止值。使用McNemar检验研究了我们人群中CK和醛缩酶对皮肌炎或多发性肌炎诊断的特异性。
结果:总CK的ROC曲线下面积(AUC)为0.716(95CI:0.651-0.775),男性CK为0.703(95CI:0.592-0.799),女性CK为0.719(95CI:0.636-0.793)。对于醛缩酶,AUC为0.505(95CI:0.437-0.573)。每次测定的优化截止点是:男性CK为112U/L,敏感性为73.9%(95CI:51.6-89.8),特异性为49.2%(95CI:35.9-62.5);女性CK为88U/L,敏感性为75.0%(95CI:57.8-87.9),特异性为50.5%(95CI:40.4-60.6);醛缩酶为5.6U/L,敏感性为61.0%(95CI:53.2-68.8),特异性为38.8%(95CI:26.5-52.6)。关于诊断为皮肌炎或多发性肌炎的个体,根据CK和醛缩酶结果正确分类为病理性的占66.7%和44.4%,分别。McNemar检验没有发现显著差异。
结论:CK的测定提供了更好的MD诊断性能,此外,在多发性肌炎和皮肌炎的情况下,醛缩酶的测定没有显着差异。因此,单一测定CK足以进行MD筛查。
OBJECTIVE: Creatine kinase (CK) and aldolase are markers traditionally used in the study of muscle damage (MD). As CK determination is more specific to muscle damage, the demand for both determinations in routine laboratory tests would entail an extra cost.
METHODS: Retrospective observational study conducted between 2019-2020. CK and aldolase concentrations from 218 patients were studied.ROC curves were analyzed for CK and aldolase for muscle damage detection. Cut-off values were selected for both strategies. Specifity of CK and aldolase for dermatomyositis or
polymyositis diagnosis in our population was studied using the McNemar\'s test.
RESULTS: The area under the ROC curve (AUC) for total CK was 0.716 (95%CI: 0.651-0.775), for CK in males it was 0.703 (95%CI: 0.592-0.799), and for CK in females was 0.719 (95%CI: 0.636-0.793). For aldolase, AUC was 0.505 (95%CI: 0.437-0.573). Optimized cut-off points for each determination were: 112 U/L for CK in men, with a sensitivity of 73.9% (95%CI: 51.6-89.8) and a specificity of 49.2% (95%CI: 35.9-62.5); 88 U/L for CK in women, with a sensitivity of 75.0% (95%CI: 57.8-87.9) and specificity of 50.5% (95%CI: 40.4-60.6); and 5.6 U/L for aldolase, with a sensitivity of 61.0% (95%CI: 53.2-68.8) and a specificity of 38.8% (95%CI: 26.5-52.6).Regarding the individuals diagnosed with dermatomyositis or
polymyositis, 66.7% and 44.4% of them were correctly classified as pathological by CK and aldolase results, respectively. McNemar\'s test did not reveal significant differences.
CONCLUSIONS: The determination of CK offers a better diagnostic performance of MD and, in addition, does not present significant differences regarding the determination of aldolase in cases of
polymyositis and dermatomyositis. Therefore, the single determination of CK would be sufficient for MD screening.