由各种真菌病原体引起的采后腐烂是影响猕猴桃产量和品质的破坏性疾病,造成重大的年度经济损失。本研究的重点是分离菌株P3-1W,被确定为Diaportheeres,是中国红阳采后腐烂病的病原体。研究强调了细胞壁降解酶(CWDEs)是关键的致病因子。特别是,纤维素酶的酶活性,β-半乳糖苷酶,多聚半乳糖醛酸酶,果胶甲基酯酶在D.eresP3-1W感染后第二天显著达到峰值。为了全面了解这些CWDE,使用PacBio和Illumina测序技术对该菌株的基因组进行了测序.分析表明,D.eresP3-1W的基因组跨越58,489,835bp,N50为5,939,879bp,GC含量为50.7%。预测并功能注释了总共15,407个总蛋白质编码基因(PCGs)。值得注意的是,在D.eresP3-1W中鉴定出857种碳水化合物活性酶(CAZymes),521个CWDE由374个糖苷水解酶(GHs)组成,108糖酯酶(CEs)和91多糖裂解酶(PLs)。此外,221辅助活动(AAs),91糖基转移酶(GTs),检测到108个碳水化合物结合模块(CBMs)。这些发现为D.eresP3-1W的CAZymes提供了有价值的见解。
Postharvest rot caused by various fungal pathogens is a damaging disease affecting kiwifruit production and quality, resulting in significant annual economic losses. This study focused on isolating the strain P3-1W, identified as Diaporthe eres, as the causal agent of \'Hongyang\' postharvest rot disease in China. The investigation highlighted cell wall degrading enzymes (CWDEs) as crucial pathogenic factors. Specially, the enzymatic activities of cellulase, β-galactosidase,
polygalacturonase, and pectin methylesterases peaked significantly on the second day after infection of D. eres P3-1W. To gain a comprehensive understanding of these CWDEs, the genome of this strain was sequenced using PacBio and Illumina sequencing technologies. The analysis revealed that the genome of D. eres P3-1W spans 58,489,835 bp, with an N50 of 5,939,879 bp and a GC content of 50.7%. A total of 15,407 total protein-coding genes (PCGs) were predicted and functionally annotated. Notably, 857 carbohydrate-active enzymes (CAZymes) were identified in D. eres P3-1W, with 521 CWDEs consisting of 374 glycoside hydrolases (GHs), 108 carbohydrate esterase (CEs) and 91 polysaccharide lyases (PLs). Additionally, 221 auxiliary activities (AAs), 91 glycosyltransferases (GTs), and 108 carbohydrate binding modules (CBMs) were detected. These findings offer valuable insights into the CAZymes of D. eres P3-1W.