慢性伤口是一个重要的全球健康问题,在统计上影响了发达国家1-2%的人口。这些伤口对患者造成相当大的不适,并且需要花费大量的时间和资源用于治疗。在新兴的治疗方法中,含有生物活性分子的药物敷料,包括天然化合物,特别有希望。因此,这项研究的目的是开发用于伤口治疗的新型抗菌敷料。具体来说,使用静电纺丝技术制造聚己内酯膜,随后使用逐层组装技术用天然聚电解质(作为聚阳离子的壳聚糖和作为聚阴离子的麦卢卡蜂蜜与精油纳米乳液的混合物)涂覆。通过QCM-D进行物理化学和形态表征,FTIR-ATR,XPS,和SEM分析。SEM和QCM-D的结果表明成功的层沉积和涂层形成。此外,FTIR-ATR和XPS分析区分不同的涂料组合物。在成纤维细胞的存在下测试涂覆的膜,证明编码VEGF的基因的生物相容性和表达,COL1和TGF-β1与愈合过程相关(通过RT-qPCR分析评估)。最后,膜对金黄色葡萄球菌和铜绿假单胞菌均表现出优异的抗菌活性,当加入肉桂精油纳米乳液时,观察到更高的细菌菌株抑制作用。一起来看,这些结果证明了纳米涂层膜在生物医学应用中的潜在应用,如伤口愈合。
Chronic wounds represent a significant global health concern, statistically impacting 1-2% of the population in developed countries throughout their lifetimes. These wounds cause considerable discomfort for patients and necessitate substantial expenditures of time and resources for treatment. Among the emerging therapeutic approaches, medicated dressings incorporating bioactive molecules, including natural compounds, are particularly promising. Hence, the objective of this study was to develop novel antimicrobial dressings for wound treatment. Specifically, polycaprolactone membranes were manufactured using the electrospinning technique and subsequently coated with natural
polyelectrolytes (chitosan as a polycation and a mixture of manuka honey with essential oils nanoemulsions as a polyanion) employing the Layer-by-Layer assembly technique. Physico-chemical and morphological characterization was conducted through QCM-D, FTIR-ATR, XPS, and SEM analyses. The results from SEM and QCM-D demonstrated successful layer deposition and coating formation. Furthermore, FTIR-ATR and XPS analyses distinguished among different coating compositions. The coated membranes were tested in the presence of fibroblast cells, demonstrating biocompatibility and expression of genes coding for VEGF, COL1, and TGF-β1, which are associated with the healing process (assessed through RT-qPCR analysis). Finally, the membranes exhibited excellent antibacterial activity against both Staphylococcus aureus and Pseudomonas aeruginosa, with higher bacterial strain inhibition observed when cinnamon essential oil nanoemulsion was incorporated. Taken together, these results demonstrate the potential application of nanocoated membranes for biomedical applications, such as wound healing.