背景:温度传感通常用于即时护理(POC)检测技术,然而,使用的便携性和便利性经常受到热敏过程和信号转导的复杂性的影响。尤其是,反应容器中的多步骤目标识别反应和温度测量在检测设备的稳定性和集成方面提出了挑战。为了在一个测定中进一步结合光热反应和信号读出,这两个过程可以集成到微型化的微流控芯片中,从而促进光热感测并实现简单的视觉温度感测作为POC检测。
结果:集成到基于微流体距离的分析设备(μDAD)上的铜离子(Cu2)催化的光热传感系统,启用视觉,便携式,以及对多个目标的灵敏定量检测,包括抗坏血酸,谷胱甘肽,碱性磷酸酶(ALP)。聚多巴胺纳米粒子(PDANP)是通过氧化还原或配位反应调节游离Cu2+合成的,促进不同的光热响应信号的转导,并提供通用的Cu2响应传感系统。通过与光热μDAD集成促进,该系统结合了PDA的光热响应和碳酸氢铵的热敏气体生产,以提高ALP检测的灵敏度,达到9.1mU/L的检测限该系统成功实现了ALP的片上检测,抗干扰能力强,回收率从96.8%到104.7%,同时相对标准偏差低于8.0%。
■μDAD设计同时适应了PDANP的光热反应和热敏气体产生反应,实现了视觉距离信号的快速感知。基于μDAD的Cu2催化的光热传感系统具有在生化分析和临床诊断中应用的巨大潜力。强调了广泛的生物标志物的多功能Cu2+调节机制。
BACKGROUND: Temperature sensing is commonly used in point-of-care (POC) detection technologies, yet the portability and convenience of use are frequently compromised by the complexity of thermosensitive processes and signal transduction. Especially, multi-step target recognition reactions and temperature measurement in the reaction vessel present challenges in terms of stability and integration of detection devices. To further combine photothermal reaction and signal readout in one assay, these two processes enable to be integrated into miniaturized microfluidic chips, thereby facilitating photothermal sensing and achieving a simple visual temperature sensing as POC detection.
RESULTS: A copper ion (Cu2+)-catalyzed photothermal sensing system integrated onto a microfluidic distance-based analytical device (μDAD), enabling the visual, portable, and sensitive quantitative detection of multiple targets, including ascorbic acid, glutathione, and alkaline phosphatase (ALP). The
polydopamine nanoparticles (PDA NPs) were synthesized by the regulation of free Cu2+ through redox or coordination reactions, facilitating the transduction of distinct photothermal response signals and providing the versatile Cu2+-responsive sensing systems. Promoted by integration with a photothermal μDAD, the system combines PDA\'s photothermal responsiveness and thermosensitive gas production of ammonium bicarbonate for improved sensitivity of ALP detection, reaching the detection limit of 9.1 mU/L. The system has successfully achieved on-chip detection of ALP with superior anti-interference capability and recoveries ranging from 96.8 % to 104.7 %, alongside relative standard deviations below 8.0 %.
UNASSIGNED: The μDAD design accommodated both the photothermal reaction of PDA NPs and thermosensitive gas production reaction, achieving the rapid sensing of visual distance signals. The μDAD-based Cu2+-catalyzed photothermal sensing system holds substantial potential for applications in biochemical analysis and clinical diagnostics, underscored by the versatile Cu2+ regulation mechanism for a broad spectrum of biomarkers.