本研究旨在开发和评估用于局部眼部递送泼尼松龙(PRD)的热响应原位微凝胶(PRD微凝胶),以提高药物生物利用度并延长眼部药物停留时间。制备PRD微乳剂(PRD-ME)的脂质纳米系统,并在0.25-0.75%的药物浓度下进行评估。通过将PRD-ME掺入10和12%Pluronic®F127(F127)或12%F127和1-10%Kolliphor®P188(F68)的组合中来制备PRD微凝胶。PRD微凝胶的物理化学特征,流变学,和粘膜粘附特性,眼睛刺激,和稳定性。结果显示PRD-ME清晰,混溶,热力学稳定,和球形,液滴尺寸(16.4±2.2nm),多分散指数(0.24±0.01),和zeta电位(-21.03±1.24mV)。PRD微凝胶澄清,pH值(5.37-5.81),表面张力(30.96-38.90mN/m),尺寸,和zeta电位的混合聚合物胶束(20.1-23.9nm和-1.34至-10.25mV,分别),相变温度(25.3-36°C),和胶凝时间(1.44-2.47分钟)。FTIR光谱揭示了PRD和微凝胶组分之间的化学相容性。PRD微凝胶显示假塑性流动,粘弹性和粘膜粘附特性,没有眼睛刺激,和药物含量(99.3至106.3%),药物持续释放16-24小时。微凝胶在物理化学和流变学上稳定三到六个月。因此,PRD微凝胶具有用于局部眼部递送的潜在载体。
This study aimed to develop and evaluate thermoresponsive in situ microgels for the local ocular delivery of prednisolone (PRD) (PRD microgels) to improve drug bioavailability and prolong ocular drug residence time. Lipid nanosystems of PRD microemulsions (PRD-MEs) were prepared and evaluated at a drug concentration of 0.25-0.75%. PRD microgels were prepared by incorporating PRD-MEs into 10 and 12% Pluronic® F127 (F127) or combinations of 12% F127 and 1-10% Kolliphor®P188 (F68). PRD microgels were characterized for physicochemical, rheological, and mucoadhesive properties, eye irritation, and stability. Results showed that PRD-MEs were clear, miscible, thermodynamically stable, and spherical with droplet size (16.4 ± 2.2 nm), polydispersity index (0.24 ± 0.01), and zeta potential (-21.03 ± 1.24 mV). The PRD microgels were clear with pH (5.37-5.81), surface tension (30.96-38.90 mN/m), size, and zeta potential of mixed polymeric micelles (20.1-23.9 nm and -1.34 to -10.25 mV, respectively), phase transition temperature (25.3-36 °C), and gelation time (1.44-2.47 min). The FTIR spectra revealed chemical compatibility between PRD and microgel components. PRD microgels showed pseudoplastic flow, viscoelastic and mucoadhesive properties, absence of eye irritation, and drug content (99.3 to 106.3%) with a sustained drug release for 16-24 h. Microgels were physicochemically and rheologically stable for three to six months. Therefore, PRD microgels possess potential vehicles for local ocular delivery.