这项研究考察了制造业,表征,和铂纳米颗粒的生物学评估,由阴沟肠杆菌合成,并用牛血清白蛋白(BSA)和白藜芦醇(RSV)包被。从深黄色到黑色的颜色变化证实了PtNP的形成,这是由于阴沟肠杆菌对氯化铂的生物还原。BSA和RSV功能化增强了这些纳米颗粒的生物相容性和治疗潜力。TGA,SEM,XRD,和FTIR用于表征,其中通过FTIR研究了PtNP和药物缀合相关官能团。XRD证实了PtNP和Pt-BSA-RSVNP的结晶性质,而TGA和SEM显示了热稳定性和药物涂层后的形态变化。在溶血测试中还发现设计的复合材料具有生物相容性,表明它们在生物医学应用中的潜力。在确认基于PtNPs的纳米焦糖石合成后,他们接受了抗菌检查,抗氧化剂,抗炎,和抗癌特性。Pt-BSA-RSVNP显示出更高的浓度依赖性DPPH清除活性,测量抗氧化能力。酶抑制试验证明对COX-2和15-LOX酶有相当大的抗炎活性。在体外抗癌研究中,Pt-BSA-RSVNP有效杀死人卵巢癌细胞。这种现象被证明是由癌症的酸性环境促进的,因为药物释放测定证实了在酸性环境中RSV从NP制剂中的释放。最后,分子对接还证明RSV作为抗氧化剂具有很强的潜力,抗菌,抗炎,和抗癌剂。总的来说,在目前的研究中,硅和体外研究表明,设计的纳米复合材料具有良好的药用应用,然而,必须进行进一步的体内实验以验证我们的发现.
This study examines the manufacturing, characterization, and biological evaluation of platinum nanoparticles, which were synthesized by Enterobacter cloacae and coated with Bovine Serum Albumin (BSA) and Resveratrol (RSV). The formation of PtNPs was confirmed with the change of color from dark yellow to black, which was due to the bioreduction of platinum chloride by E. cloacae. BSA and RSV functionalization enhanced these nanoparticles\' biocompatibility and therapeutic potential. TGA, SEM, XRD, and FTIR were employed for characterization, where PtNPs and drug conjugation-related functional groups were studied by FTIR. XRD confirmed the crystalline nature of PtNPs and Pt-BSA-RSV NPs, while TGA and SEM showed thermal stability and post-drug coating morphological changes. Designed composite was also found to be biocompatible in nature in hemolytic testing, indicating their potential in Biomedical applications. After confirmation of PtNPs based nanocaompsite synthesis, they were examined for anti-bacterial, anti-oxidant, anti-inflammatory, and anti-cancer properties. Pt-BSA-RSV NPs showed higher concentration-dependent DPPH scavenging activity, which measured antioxidant capability. Enzyme inhibition tests demonstrated considerable anti-inflammatory activity against COX-2 and 15-LOX enzymes. In in vitro anticancer studies, Pt-BSA-RSV NPs effectively killed human ovarian cancer cells. This phenomenon was demonstrated to be facilitated by the acidic environment of cancer, as the drug release assay confirmed the release of RSV from the NP formulation in the acidic environment. Finally, Molecular docking also demonstrated that RSV has strong potential as an anti-oxidant, antibacterial, anti-inflammatory, and anticancer agent. Overall, in silico and in vitro investigations in the current study showed good medicinal applications for designed nanocomposites, however, further in-vivo experiments must be conducted to validate our findings.