功能,稳定性,质膜(PM)蛋白的更新对于细胞稳态至关重要。与可溶性蛋白质相比,质膜蛋白的质量控制极具挑战性。不符合高质量控制标准对细胞和生物体健康有害。J域蛋白(JDPs)是与其他伴侣和蛋白质降解机制合作以监督细胞蛋白质质量控制(PQC)的最多样化的伴侣之一。虽然支离破碎,来自不同模型的可用文献,包括酵母,哺乳动物,和植物,表明JDP帮助PM蛋白合成,折叠,贩运到他们的目的地以及他们的退化,通过胞吞或蛋白酶体降解途径。此外,一些JDP直接与膜相互作用以调节PM处的蛋白质的稳定性和/或功能性。出现的去卷积图片是PM蛋白在整个生命周期中从一个JDP传递到另一个JDP,进一步强调了Hsp70:JDP机器在细胞中的多功能性。
The function, stability, and turnover of plasma membrane (PM) proteins are crucial for cellular homeostasis. Compared to soluble proteins, quality control of plasma membrane proteins is extremely challenging. Failure to meet the high quality control standards is detrimental to cellular and organismal health. J-domain proteins (JDPs) are among the most diverse group of chaperones that collaborate with other chaperones and protein degradation machinery to oversee cellular protein quality control (PQC). Although fragmented, the available literature from different models, including yeast, mammals, and plants, suggests that JDPs assist PM proteins with their synthesis, folding, and trafficking to their destination as well as their degradation, either through endocytic or proteasomal degradation pathways. Moreover, some JDPs interact directly with the membrane to regulate the stability and/or functionality of proteins at the PM. The deconvoluted picture emerging is that PM proteins are relayed from one JDP to another throughout their life cycle, further underscoring the versatility of the Hsp70:JDP machinery in the cell.