目标:淫羊藿。是淫羊藿属的多年生持久性C3植物。在小檗科表现出严重的生理和形态种子休眠。我们将成熟的E.brevicornu种子置于9种分层处理条件下,并通过种子胚生长状态评估与相关代谢途径和基因共表达分析相结合,探索其影响机制。
结果:我们通过冷却单元(CU)模型确定了3.9°C是E.brevicornu种子的最佳冷分层温度。最佳处理是变温分层(10/20°C,12/12h)持续4个月,然后进行低温分层(4°C)持续3个月(4-3)。在9个处理(0-0、0-3、1-3、2-3、3-3、4-3、4-3、4-2、4-2、4-1、4-0)中,总共63801个差异表达基因注释为17个簇中的2587个转录因子(TFs)。在休眠释放处理组中特异性高表达的基因在种子休眠和脂肪酸降解的胚胎发育中显著富集,说明了这两个过程的重要性。共表达分析表明,TFGRF与基因有最多的相互关系,以ZF-HD和YABBY以及MYB为中心的多种相互作用,GRF,并观察到TCP。
结论:在这项研究中,植物激素信号途径和脂肪酸降解途径的分析揭示了关键基因在休眠释放期间的变化。为筛选与种子休眠相关的基因提供了依据。
OBJECTIVE: Epimedium brevicornu Maxim. is a perennial persistent C3 plant of the genus Epimedium Linn. in the family Berberaceae that exhibits severe physiological and morphological seed dormancy.We placed mature E. brevicornu seeds under nine stratification treatment conditions and explored the mechanisms of influence by combining seed embryo growth status assessment with related metabolic pathways and gene co-expression analysis.
RESULTS: We identified 3.9 °C as the optimum cold-stratification temperature of E. brevicornu seeds via a chilling unit (CU) model. The best treatment was variable-temperature stratification (10/20 °C, 12/12 h) for 4 months followed by low-temperature stratification (4 °C) for 3 months (4-3). A total of 63801 differentially expressed genes were annotated to 2587 transcription factors (TFs) in 17 clusters in nine treatments (0-0, 0-3, 1-3, 2-3, 3-3, 4-3, 4-2, 4-1, 4-0). Genes specifically highly expressed in the dormancy release treatment group were significantly enriched in embryo development ending in seed dormancy and fatty acid degradation, indicating the importance of these two processes. Coexpression analysis implied that the TF GRF had the most reciprocal relationships with genes, and multiple interactions centred on zf-HD and YABBY as well as on MYB, GRF, and TCP were observed.
CONCLUSIONS: In this study, analyses of plant hormone signal pathways and fatty acid degradation pathways revealed changes in key genes during the dormancy release of E. brevicornu seeds, providing evidence for the filtering of E. brevicornu seed dormancy-related genes.