马尾松羔羊.是我国分布最广、造林面积最大的木材树种,提供大量木材,松节油和生态产品。但低温限制了其生长和地理分布。生理和分子研究可以很好地解释马尾松对低温的反应机制。在这项研究中,生理生化指标,细胞形态学,木质素含量,基因调控网络,从生理、生物化学,和分子观点。结果表明,在低温胁迫下,耐寒品种保持了较高的渗透调节物质含量,根形态和结构保持完整。在低温胁迫的初始阶段,差异表达基因为7148个,随着胁迫时间的延长,差异表达基因的数量减少到1991年。马尾松假单胞菌可能通过调节苯丙烷代谢来指导其对低温的反应,淀粉和蔗糖代谢,激素信号通路,和转录因子。BAM,4CL,CCoAOMT,PRX5,WRKYs,与激素合成相关的基因起着重要作用。马尾藻品种的反应机制可能有所不同。在这项研究中,利用生理和分析技术研究了马尾松根尖对低温胁迫的响应机制。本研究结果为深入研究低温胁迫条件下马尾松的分子功能奠定了基础。
Pinus massoniana Lamb. is the timber species with the widest distribution and the largest afforestation area in China, providing a large amount of timber, turpentine and ecological products. but low temperature limits its growth and geographical distribution. Physiological and molecular studies can well explain the mechanism of P. massoniana response to low temperature. In this study, physiological and biochemical indexes, cell morphology, lignin content, gene regulatory networks, and gene expression patterns of different P. massoniana varieties (cold-tolerant and cold-sensitive) were studied from physiological, biochemical, and molecular perspectives. The results indicated that under low-temperature stress, the cold-tolerant cultivar maintained high contents of osmoregulatory substances, and the root morphology and structure remained intact. In the initial stage of low-temperature stress, the number of differentially expressed genes was 7148, and with the extension of stress time, the number of differentially expressed genes decreased to 1991. P. massoniana might direct its responses to low temperature by regulating phenylpropane metabolism, starch and sucrose metabolism, hormone signaling pathways, and transcription factors. BAM, 4CL, CCoAOMT, PRX5, WRKYs, and hormone synthesis related genes play important roles. P. massoniana cultivars may vary in response mechanisms. In this study, physiological and analytical techniques were used to study the root tip response mechanism of Masson\'s pine to low temperature stress. The results of this study lay a foundation for in-depth research on the molecular functions of P. massoniana under low-temperature stress conditions.