在先前的分子系统发育研究中,认识到了东亚(或Physospermophis)进化枝,该研究涉及了Apioideae亚科Apioideae的高级关系。这个进化枝的组成,其组成分类群之间的系统发育关系,先前被确定有问题的物种的位置尚未解决。在这里,获得了150种Apioideae的nrDNAITS序列,代表分布在东亚的物种或在东亚进化枝中包含一个或多个物种的属。这些数据,连同已发表的其他Apioideae的ITS序列(共3678种),进行了最大似然和贝叶斯推断分析。结果表明,东亚进化枝包含11个目前公认的属的代表:汉森尼亚,膜虫,Keraymonia,Sinolimprichtia,Acronema,膜状,Physosopermopsis,Pimpinella,中心,Tongoloa,和Trachyum。然而,后七个属的成员不属于东亚分支,包括除Tongoloa之外的所有通用类型。在进化枝内部,组成这七个属的物种广泛混合,比以前意识到的关系之间的混淆大大增加。有问题的物种Physospermopsiscuneata被证实属于东亚进化枝,而P.rubrinervis在胸膜部落中与一般类型结盟。金顿-沃迪植物被确认为植物植物属的成员,而cuneata和Tongoloastewardii的通用归因尚不清楚。两种Sinocarum(S.filicinum和S.wolffianum)被转移到Meeboldia属中。这是迄今为止对东亚进化枝进行的最全面的分子系统发育研究,虽然结果增加了对进化枝的系统理解,他们还强调需要对Apioideae中最难以分类的群体之一进行进一步研究。
The East Asia (or Physospermopsis) clade was recognized in previous molecular phylogenetic investigations into the higher-level relationships of Apiaceae subfamily Apioideae. The composition of this clade, the phylogenetic relationships among its constituent taxa, and the placement of species previously determined to be problematic have yet to be resolved. Herein, nrDNA ITS sequences were obtained for 150 accessions of Apioideae, representing species whose distributions are in East Asia or genera having one or more species included within the East Asia clade. These data, along with published ITS sequences from other Apioideae (for 3678 accessions altogether), were subjected to maximum likelihood and Bayesian inference analyses. The results show that the East Asia clade contains representatives of 11 currently recognized genera: Hansenia, Hymenolaena, Keraymonia, Sinolimprichtia, Acronema, Hymenidium, Physospermopsis,
Pimpinella, Sinocarum, Tongoloa, and Trachydium. However, the latter seven genera have members falling outside of the East Asia clade, including the generic types of all except Tongoloa. Within the clade, the species comprising these seven genera are widely intermingled, greatly increasing confusion among relationships than previously realized. The problematic species Physospermopsis cuneata is confirmed as falling within the East Asia clade, whereas P. rubrinervis allies with the generic type in tribe Pleurospermeae. Physospermopsis kingdon-wardii is confirmed as a member of the genus Physospermopsis, whereas the generic attributions of P. cuneata and Tongoloa stewardii remain unclear. Two species of Sinocarum (S. filicinum and S. wolffianum) are transferred into the genus Meeboldia. This is the most comprehensive molecular phylogenetic investigation of the East Asia clade to date, and while the results increase systematic understanding of the clade, they also highlight the need for further studies of one of the most taxonomically intractable groups in Apioideae.