背景口咽念珠菌属物种是健康个体口腔中的部分共生微生物区系。共生念珠菌可以成为机会主义者,并通过生态线索和毒力因子的表达在免疫力受损的个体中过渡到口咽念珠菌病(OPC)的致病原因。有限的研究已经评估了乌干达抗逆转录病毒疗法(ART)中患有人类免疫缺陷病毒(PLHIV)的人中口咽念珠菌的毒力属性。目的评价人免疫缺陷病毒伴口咽念珠菌病抗逆转录病毒治疗口咽念珠菌的毒力特性方法从样本库中检索35(35)株PLHIV伴OPC抗逆转录病毒治疗口咽念珠菌,采用卵黄琼脂法进行磷脂酶活性评价,使用牛血清白蛋白琼脂法的蛋白酶活性,使用血琼脂平板法的溶血素活性,酯酶活性使用吐温80不透明度测试培养基方法,使用经典管法的凝固酶活性和使用体外微量滴定板测定法的生物膜形成。结果在33/35(94.3%)和31/35(88.6%)菌株中检测到磷脂酶和蛋白酶活性,分别。多达25/35(71.4%)的菌株表现出生物膜形成,而酯酶活性在23/35(65.7%)的菌株中得到证实。在18/35(51.4%)中检测到的菌株中,产生溶血素和凝固酶的分离株较少(60%)。结论磷脂酶和蛋白酶活性是口咽念珠菌中最强的毒力属性。
UNASSIGNED: Oropharyngeal Candida species are part commensal microflora in the the oral cavity of health individuals. Commensal Candida species can become opportunist and transition to pathogenic causes of oropharyngeal candidiasis (OPC) in individuals with impaired immunity through ecological cues and expression of virulence factors. Limited studies have evaluated virulence attributes of oropharyngeal Candida species among people living with human immunodeficiency virus (PLHIV) with OPC on antiretroviral therapy (ART) in Uganda.
UNASSIGNED: Evaluation of the Virulence Attributes of Oropharyngeal Candida Species Isolated from People Living with Human Immunodeficiency Virus with Oropharyngeal Candidiasis on Antiretroviral Therapy.
UNASSIGNED: Thirty-five (35) Candida isolates from PLHIV with OPC on ART were retrieved from sample repository and evaluated for
phospholipase activity using the egg yolk agar method, proteinase activity using the bovine serum albumin agar method, hemolysin activity using the blood agar plate method, esterase activity using the Tween 80 opacity test medium method, coagulase activity using the classical tube method and biofilm formation using the microtiter plate assay method in vitro.
UNASSIGNED: Phospholipase and proteinase activities were detected in 33/35 (94.3%) and 31/35 (88.6%) of the strains, respectively. Up to 25/35 (71.4%) of the strains exhibited biofilm formation while esterase activity was demonstrated in 23/35 (65.7%) of the strains. Fewer isolates 21/35 (60%) of the strains produced hemolysin and coagulase production was the least virulence activity detected in 18/35 (51.4%).
UNASSIGNED: Phospholipase and proteinase activities were the strongest virulence attributes of oropharyngeal Candida species.