快速的工业化和城市化已导致镉(Cd)严重的土壤污染,因此需要有效的修复策略。植物修复是一种广泛采用的修复Cd污染土壤的技术。先前的研究表明,Abelmoschusmanihot具有较高的Cd积累能力和耐受性,表明其在Cd土壤修复中的潜力。然而,其对Cd胁迫的响应机制尚不清楚。在这项研究中,生理,转录组,进行了代谢组学分析,以探讨A.manihot根在不同时间点对Cd胁迫的响应。结果表明,Cd胁迫显着增加了A.manihot的丙二醛(MDA)水平,同时激活了它的抗氧化防御系统,提高超氧化物歧化酶(SOD)的活性,过氧化物酶(POD),过氧化氢酶(CAT)下降19.73%-50%,22.87%-38.89%,12小时时32.31%-45.40%,36h,72小时,7天,分别,与对照组(CK)相比。此外,转录组学和代谢组学分析揭示了245、5,708、9,834和2,323个差异表达基因(DEG),在12小时与66、62、156和90种差异表达的代谢物(DEM)一起,36h,72小时,7天,分别。通过加权基因共表达网络(WGCNA)分析生理指标和转录本表达,涉及苯丙烷生物合成的八个hub基因,信号转导,并确定了金属运输。此外,代谢组学和转录组学数据的综合分析强调了Cd胁迫下脂质代谢和苯丙素生物合成途径的激活,表明这些途径在A.manihot的解毒过程和增强Cd耐受性中起着至关重要的作用。这项全面的研究为A.manihot对Cd毒性的响应机制提供了详细的见解。
Rapid industrialization and urbanization have caused severe soil contamination with cadmium (Cd) necessitating effective remediation strategies. Phytoremediation is a widely adopted technology for remediating Cd-contaminated soil. Previous studies have shown that Abelmoschus manihot has a high Cd accumulation capacity and tolerance indicating its potential for Cd soil remediation. However, the mechanisms underlying its response to Cd stress remain unclear. In this study, physiological, transcriptomic, and metabolomic analyses were conducted to explore the response of A. manihot roots to Cd stress at different time points. The results revealed that Cd stress significantly increased malondialdehyde (MDA) levels in A. manihot, which simultaneously activated its antioxidant defense system, enhancing the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) by 19.73%-50%, 22.87%-38.89%, and 32.31%-45.40% at 12 h, 36 h, 72 h, and 7 days, respectively, compared with those in the control (CK). Moreover, transcriptomic and metabolomic analyses revealed 245, 5,708, 9,834, and 2,323 differentially expressed genes (DEGs), along with 66, 62, 156, and 90 differentially expressed metabolites (DEMs) at 12 h, 36 h, 72 h, and 7 days, respectively. Through weighted gene coexpression network analysis (WGCNA) of physiological indicators and transcript expression, eight hub genes involved in
phenylpropanoid biosynthesis, signal transduction, and metal transport were identified. In addition, integrative analyses of metabolomic and transcriptomic data highlighted the activation of lipid metabolism and
phenylpropanoid biosynthesis pathways under Cd stress suggesting that these pathways play crucial roles in the detoxification process and in enhancing Cd tolerance in A. manihot. This comprehensive study provides detailed insights into the response mechanisms of A. manihot to Cd toxicity.