新型精神活性物质(NPS)是一种滥用药物,旨在模仿目前已知的非法药物的作用,其结构不断变化以逃避监视。因此,快速识别社区中的NPS使用需要立即采取行动。本研究旨在开发一种使用LC-HRMS鉴定废水样品中NPS的目标和可疑筛查方法。使用参考标准建立了95个传统和NPS的内部数据库,并开发了一种分析方法。从韩国的29个污水处理厂(WWTP)收集了废水样本,占总人口的50%。使用内部数据库筛选了废水样品中的精神活性物质,并开发了分析方法。在目标分析中总共检测到14种物质,包括三个NPS(N-甲基-2-AI,25E-NBOMe,和25D-NBOMe)和11种传统精神活性物质及其代谢产物(唑吡坦苯基-4-COOH,麻黄碱,利醋甲酸,曲马多,苯甲拉嗪,苯丙胺嗪,芬特明,甲基苯丙胺,可待因,吗啡,和氯胺酮)。在这些中,N-甲基-2-AI,唑吡坦苯基-4-COOH,麻黄碱,利醋甲酸,曲马多,苯甲拉嗪,检测频率超过50%。首先,在所有废水样品中均检测到N-甲基-2-Al。此外,四种NPSs(苯丙胺-N-丙基,苄达明,异乙卡西酮,甲氧基苯那敏)在可疑筛查分析中被初步鉴定为2b级。这是在国家一级使用目标和可疑分析方法调查NPS的最全面的研究。这项研究提出了在韩国持续监测NPS的必要性。
New psychoactive substances (NPS) are a type of abused drug designed to mimic the effects of the currently known illicit drugs, whose structures are constantly changing to escape surveillance. The quick identification of NPS use in the community therefore demands immediate action. This study aimed to develop a target and suspect screening method using LC-HRMS to identify NPS in wastewater samples. An in-house database of 95 traditional and NPS was built using the reference standards, and an analytical method was developed. Wastewater samples were collected from 29 wastewater treatment plants (WWTP) across South Korea, representing 50 % of the total population. The psychoactive substances in waste water samples were screened using in-house database and developed analytical methods. A total of 14 substances were detected in the target analysis, including three NPS (N-methyl-2-AI, 25E-NBOMe, and 25D-NBOMe) and 11 traditional psychoactive substances and their metabolites (zolpidem phenyl-4-COOH, ephedrine, ritalinic acid, tramadol,
phenmetrazine, phendimetrazine, phentermine, methamphetamine, codeine, morphine, and ketamine). Out of these, N-methyl-2-AI, zolpidem phenyl-4-COOH, ephedrine, ritalinic acid, tramadol,
phenmetrazine, and phendimetrazine were detected with a detection frequency of over 50 %. Primarily, N-methyl-2-Al was detected in all the wastewater samples. Additionally, four NPSs (amphetamine-N-propyl, benzydamine, isoethcathinone, methoxyphenamine) were tentatively identified at level 2b in a suspect screening analysis. This is the most comprehensive study to investigate NPS using target and suspect analysis methods at the national level. This study raises a need for continuous monitoring of NPS in South Korea.