镰刀菌冠腐病,由假镰刀菌引起的,是一种影响谷类作物产量和质量的毁灭性疾病。过氧化物酶体是单膜细胞器,在真核细胞的各种生物过程中起关键作用。为了在功能上表征假草中的过氧化物酶体生物合成受体蛋白FpPEX5和FpPEX7,我们构建了缺失突变体,ΔFpPEX5和ΔFpPEX7,以及互补菌株,ΔFpPEX5-C和ΔFpPEX7-C,并使用各种表型观察分析了FpPEX5和FpPEX7蛋白的功能。FpPEX5和FpPEX7的缺失导致菌丝生长和分生孢子的显著缺陷,并阻断了过氧化物酶体靶向信号1和过氧化物酶体靶向信号2通路,参与过氧化物酶体基质蛋白转运,增加脂滴和活性氧的积累。FpPEX5和FpPEX7的缺失可以减少产毒体的形成并降低假赤霉病的致病性。这些结果表明,FpPEX5和FpPEX7在生长中起着至关重要的作用,无性繁殖,毒力,和假赤霉病的脂肪酸利用。本研究为小麦茎腐病防治提供了理论依据。
Fusarium crown rot, caused by Fusarium pseudograminearum, is a devastating disease affecting the yield and quality of cereal crops. Peroxisomes are single-membrane organelles that play a critical role in various biological processes in eukaryotic cells. To functionally characterise peroxisome biosynthetic receptor proteins FpPEX5 and FpPEX7 in F. pseudograminearum, we constructed deletion mutants, ΔFpPEX5 and ΔFpPEX7, and complementary strains, ΔFpPEX5-C and ΔFpPEX7-C, and analysed the functions of FpPEX5 and FpPEX7 proteins using various phenotypic observations. The deletion of FpPEX5 and FpPEX7 resulted in a significant deficiency in mycelial growth and conidiation and blocked the peroxisomal targeting signal 1 and peroxisomal targeting signal 2 pathways, which are involved in peroxisomal matrix protein transport, increasing the accumulation of lipid droplets and reactive oxygen species. The deletion of FpPEX5 and FpPEX7 may reduce the formation of toxigenic bodies and decrease the pathogenicity of F. pseudograminearum. These results indicate that FpPEX5 and FpPEX7 play vital roles in the growth, asexual reproduction, virulence, and fatty acid utilisation of F. pseudograminearum. This study provides a theoretical basis for controlling stem rot in wheat.