背景:已知糖尿病会损害肝脏和肾脏,导致肝功能障碍和肾衰竭。蜂蜜被认为有助于降低糖尿病患者的血糖水平并减少糖尿病并发症。然而,无刺蜜蜂蜂蜜(SBH)在减轻糖尿病肝肾损害方面的作用尚未得到充分研究。
目的:观察SBH对链脲佐菌素(STZ;55mg/kg)糖尿病SpragueDawley大鼠肾脏和肝脏的影响。
方法:将大鼠分组如下(每组n=6):非糖尿病(ND),未经治疗的糖尿病(UNT),二甲双胍治疗(MET),和SBH+二甲双胍治疗(SBME)组。糖尿病诱导成功后,ND和UNT大鼠给予生理盐水,而治疗组接受SBH(2.0g/kg和/或二甲双胍(250mg/kg)治疗12d。使用苏木精和伊红(H&E)和高碘酸希夫(PAS)染色评估血清生化参数和组织学变化。
结果:关于H&E和PAS染色,ND组显示Bowman囊和小管的正常结构和细胞结构,而UNT和MET组的肾小球细胞增加,基底膜增厚。根据H&E染色,SBH处理组显示肾小球的水肿变化和轻度细胞性降低,与ND组相比,但两者在PAS染色上相似。同样,在H&E染色上,SBME治疗组肾小球细胞性增加,但在PAS染色上与SBH和ND组相当。UNT糖尿病大鼠有肾小管积水,比其他组小。SBH组肝组织脂肪液泡形成减少,血窦扩张。相反,UNT组的血糖水平很高,随后增加了MDA水平,最终导致肝脏损伤.SBH处理减少了这种伤害,空腹血糖最低,血清丙氨酸转氨酶,天冬氨酸转氨酶,和碱性磷酸酶水平与其他组相比,尽管肝酶水平无统计学意义。
结论:鲍曼胶囊的细胞密度,以及肾小管的组织学改变,肾小球膜,口服SBH后糖尿病大鼠的肝脏组织与ND大鼠相似。因此,SBH在糖尿病大鼠模型中表现出保护性肝肾作用。
BACKGROUND: Diabetes is known damage the liver and kidney, leading to hepatic dysfunction and kidney failure. Honey is believed to help in lowering the blood glucose levels of diabetic patients and reducing diabetic complications. However, the effect of stingless bee honey (SBH) administration in relieving liver and kidney damage in diabetes has not been well-studied.
OBJECTIVE: To investigate the effect of SBH administration on the kidney and liver of streptozotocin-induced (STZ; 55 mg/kg) diabetic Sprague Dawley rats.
METHODS: The rats were grouped as follows (n = 6 per group): non-diabetic (ND), untreated diabetic (UNT), metformin-treated (MET), and SBH+metformin-treated (SBME) groups. After successful diabetic induction, ND and UNT rats were given normal saline, whereas the treatment groups received SBH (2.0 g/kg and/or metformin (250 mg/kg) for 12 d. Serum biochemical parameters and histological changes using hematoxylin and eosin (H&E) and periodic acid-Schiff (PAS) staining were evaluated.
RESULTS: On H&E and PAS staining, the ND group showed normal architecture and cellularity of Bowman\'s capsule and tubules, whereas the UNT and MET groups had an increased glomerular cellularity and thickened basement membrane. The SBH-treated group showed a decrease in hydropic changes and mild cellularity of the glomerulus vs the ND group based on H&E staining, but the two were similar on PAS staining. Likewise, the SBME-treated group had an increase in cellularity of the glomerulus on H&E staining, but it was comparable to the SBH and ND groups on PAS staining. UNT diabetic rats had tubular hydropic tubules, which were smaller than other groups. Reduced fatty vacuole formation and dilated blood sinusoids in liver tissue were seen in the SBH group. Conversely, the UNT group had high glucose levels, which subsequently increased MDA levels, ultimately leading to liver damage. SBH treatment reduced this damage, as evidenced by having the lowest fasting glucose, serum alanine transaminase, aspartate transaminase, and alkaline phosphatase levels compared to other groups, although the levels of liver enzymes were not statistically significant.
CONCLUSIONS: The cellularity of the Bowman\'s capsule, as well as histological alteration of kidney tubules, glomerular membranes, and liver tissues in diabetic rats after oral SBH resembled those of ND rats. Therefore, SBH exhibited a protective hepatorenal effect in a diabetic rat model.