全氟辛烷磺酸(PFOS)是一种持久性化学物质,长期以来一直对人类健康构成威胁。然而,全氟辛烷磺酸对各种器官的分子效应没有得到很好的研究。在这项研究中,雄性Sprague-Dawley大鼠通过管饲法用各种剂量的全氟辛烷磺酸治疗21天。随后,肝脏,肺,心,肾,胰腺,睾丸,收集大鼠血清进行脂质分析。我们应用了聚焦脂质组学分析策略来识别含磷酸胆碱的脂质的关键脂质反应,包括磷脂酰胆碱和鞘磷脂。偏最小二乘判别分析显示,受全氟辛烷磺酸暴露影响最大的器官是肝脏,肾,和睾丸。大鼠脂质分布的变化表明,暴露后,肝脏中的二酰基磷脂酰胆碱和22:6磷脂酰胆碱的水平,肾,老鼠的睾丸减少了,而含有20:3的磷脂酰胆碱的水平增加。此外,含多不饱和脂肪酸的血浆胆碱水平降低。鞘磷脂水平的变化表明器官依赖性反应。肝脏中的鞘磷脂水平降低,肾脏中的非单调剂量反应,并观察到全氟辛烷磺酸暴露后睾丸的不规则反应。这些脂质反应可能与磷脂酰胆碱合成的改变有关。脂肪酸代谢,膜性质,和肝脏中的氧化应激,肾,和睾丸。肝脏中的脂质反应可能有助于观察到的肝脏与体重比的增加。研究结果表明全氟辛烷磺酸在多器官中的潜在毒性和可能的机制。
Perfluorooctane sulfonate (PFOS) is a persistent chemical that has long been a threat to human health. However, the molecular effects of PFOS on various organs are not well studied. In this study, male Sprague-Dawley rats were treated with various doses of PFOS through gavage for 21 days. Subsequently, the liver, lung, heart, kidney, pancreas, testis, and serum of the rats were harvested for lipid analysis. We applied a focusing lipidomic analytical strategy to identify key lipid responses of phosphorylcholine-containing lipids, including phosphatidylcholines and sphingomyelins. Partial least squares discriminant analysis revealed that the organs most influenced by PFOS exposure were the liver, kidney, and testis. Changes in the lipid profiles of the rats indicated that after exposure, levels of diacyl-phosphatidylcholines and 22:6-containing phosphatidylcholines in the liver, kidney, and testis of the rats decreased, whereas the level of 20:3-containing phosphatidylcholines increased. Furthermore, levels of polyunsaturated fatty acids-containing plasmenylcholines decreased. Changes in sphingomyelin levels indicated organ-dependent responses. Decreased levels of sphingomyelins in the liver, nonmonotonic dose responses in the kidney, and irregular responses in the testis after PFOS exposure are observed. These lipid responses may be associated with alterations pertaining to phosphatidylcholine synthesis, fatty acid metabolism, membrane properties, and oxidative stress in the liver, kidney, and testis. Lipid responses in the liver could have contributed to the observed increase in liver to body weight ratios. The findings suggest potential toxicity and possible mechanisms associated with PFOS in multiple organs.