■内皮糖萼是保护内皮细胞结构和功能的重要屏障。雄激素缺乏是导致内皮细胞结构和功能损害的常见因素。
■探讨低雄激素状态大鼠阴茎海绵体内皮糖萼的变化及其与勃起功能的关系。
■18只10周龄Sprague-Dawley雄性大鼠随机分为3组(每组6只):假手术,阉割,去势+睾酮替代。造模4周后测量阴茎的最大海绵体内压/平均动脉压。内皮型一氧化氮合酶(eNOS)的表达水平,磷酸-eNOS,syndecan1乙酰肝素酶,阴茎海绵体组织中的一氧化氮和血清硫酸乙酰肝素水平,透明质酸,肿瘤坏死因子α,和白细胞介素6测定。用透射电镜观察阴茎组织内皮糖萼的超微结构。
■去势大鼠阴茎海绵体内皮糖萼厚度明显低于对照组。
■在去势大鼠中,内皮糖萼厚度,syndecan1级,磷酸化eNOS与eNOS的比例,一氧化氮水平,和最大海绵体内压/平均动脉压(3V,5V)均显著低于假手术组(P<.05)。去势组乙酰肝素酶表达、血清肿瘤坏死因子α和白细胞介素6水平明显高于假手术组(P<0.05)。
■上调阴茎海绵体中内皮糖萼的表达可能是治疗低雄激素水平引起的勃起功能障碍的新方法。
■这项研究证实,低雄激素状态促进内皮糖萼的分解。然而,需要进一步的研究来确定雄激素是否与内皮糖萼的合成有关。
■低雄激素状态可能通过内皮糖萼受损抑制阴茎海绵体组织中的一氧化氮水平,导致大鼠勃起功能受到抑制。
UNASSIGNED: The endothelial glycocalyx is an important barrier that protects the structure and function of endothelial cells. Androgen deficiency is a common factor that causes structural and functional impairment of endothelial cells.
UNASSIGNED: To investigate changes in the endothelial glycocalyx in the penile corpus cavernosum of the rat with low androgen status and its relationship with erection function.
UNASSIGNED: Eighteen 10-week-old Sprague-Dawley male rats were randomly divided into 3 groups (n = 6 each): sham operation, castration, and castration + testosterone replacement. The maximum intracavernosal pressure/mean arterial pressure of the
penis was measured after modeling for 4 weeks. The expression levels of endothelial nitric oxide synthase (eNOS), phospho-eNOS, syndecan 1, heparanase, and nitric oxide in penile cavernous tissue and the serum levels of heparan sulfate, hyaluronic acid, tumor necrosis factor α, and interleukin 6 were determined. Transmission electron microscopy was used to observe the ultrastructure of the endothelial glycocalyx in penile tissue.
UNASSIGNED: The thickness of the endothelial glycocalyx in the penile corpus cavernosum of castrated rats was significantly lower than that of the control group.
UNASSIGNED: In the castrated rats, the endothelial glycocalyx thickness, syndecan 1 level, ratio of phospho-eNOS to eNOS, nitric oxide level, and maximum intracavernosal pressure/mean arterial pressure (3 V, 5 V) were significantly lower than those in the sham group (P < .05). The expression of heparanase and the serum levels of tumor necrosis factor α and interleukin 6 were significantly higher in the castrated group than in the sham group (P < .05).
UNASSIGNED: Upregulating the expression of the endothelial glycocalyx in the penile corpus cavernosum may be a new method for treating erectile dysfunction caused by low androgen levels.
UNASSIGNED: This study confirms that low androgen status promotes the breakdown of the endothelial glycocalyx. However, further research is needed to determine whether androgens are related to the synthesis of the endothelial glycocalyx.
UNASSIGNED: Low androgen status may suppress the level of nitric oxide in the cavernous tissue of the
penis via impairment of the endothelial glycocalyx, resulting in inhibited erection function in rats.