到现在,在农业实践中,尚未实施有效的植物保护方法,以抵抗来自鸡杆菌科的经济上重要的细菌植物病原体。在这个观点中,我们对反应排放系统的操作参数进行了多变量优化,采用直流大气压辉光放电,与流动的液体阴极(FLC-dc-APGD)接触产生,用于生产具有定义的物理化学和抗植物致病特性的血浆活化液体(PAL)。因此,评估了操作参数对在这些条件下获得的PAL电导率的影响。揭示的最佳操作条件,在此条件下获得最高电导率的PAL,如下:溶液的流速等于2.0mLmin-1,放电电流为30mA,和无机盐浓度(硝酸铵,溶液中的NH4NO3)为0.50%(m/w)。开发的PAL对solaniDickeyaIFB0099和嗜酸杆菌IFB5103菌株具有抑菌和杀菌特性,最小抑制和最小杀菌浓度等于25%。暴露于25%PAL24小时后,100%(1-2×106)的枯萎病菌和食腐疫病菌细胞丧失活力。我们将PAL的抗菌特性归因于深层穿透性的存在,活性氧和氮(RONS),是,在这种情况下,OH,O,O3、H2O2、HO2、NH、N2,N2+,NO2-,NO3-,NH4+。推定,产生的低成本,环保,易于存储,和运输PAL,具有所需的抗菌和物理化学性质,可以在植物保护领域找到许多应用。
To the present day, no efficient plant protection method against economically important bacterial phytopathogens from the Pectobacteriaceae family has been implemented into agricultural practice. In this view, we have performed a multivariate optimization of the operating parameters of the reaction-discharge system, employing direct current atmospheric pressure glow discharge, generated in contact with a flowing liquid cathode (FLC-dc-APGD), for the production of a plasma-activated liquid (PAL) of defined physicochemical and anti-phytopathogenic properties. As a result, the effect of the operating parameters on the conductivity of PAL acquired under these conditions was assessed. The revealed optimal operating conditions, under which the PAL of the highest conductivity was obtained, were as follows: flow rate of the solution equaled 2.0 mL min-1, the discharge current was 30 mA, and the inorganic salt concentration (ammonium nitrate, NH4NO3) in the solution turned out to be 0.50% (m/w). The developed PAL exhibited bacteriostatic and bactericidal properties toward Dickeya solani IFB0099 and Pectobacterium atrosepticum IFB5103 strains, with minimal inhibitory and minimal bactericidal concentrations equaling 25%. After 24 h exposure to 25% PAL, 100% (1-2 × 106) of D. solani and P. atrosepticum cells lost viability. We attributed the antibacterial properties of PAL to the presence of deeply penetrating, reactive oxygen and nitrogen species (RONS), which were, in this case, OH, O, O3, H2O2, HO2, NH, N2, N2+, NO2-, NO3-, and NH4+. Putatively, the generated low-cost, eco-friendly, easy-to-store, and transport PAL, exhibiting the required antibacterial and physicochemical properties, may find numerous applications in the plant protection sector.