光周期是生活在温带和极地纬度的生物中季节性响应的主要环境驱动因素。其他外部线索,如食物和温度,和包括荷尔蒙在内的内部线索,干预以微调生理功能到太阳年的相位。在哺乳动物中,中底下丘脑(MBH)是这些线索的关键整合者,它协调了各种各样的季节性函数,包括繁殖。这里,使用RNAseq和RT-qPCR,我们证明了先前在母羊中鉴定的光周期反应的分子成分在does中广泛保守(雌性山羊,Caprahircus),有50个基因的共同核心。这个核心组可以定义为“MBH季节性转录组”,其中包括控制三碘甲状腺原氨酸(T3)生产的MBH内光周期开关的结节-短小胶质细胞神经内分泌逆行途径的关键参与者(Tshb,Eya3、Dio2和SlcO1c1),两种组蛋白甲基转移酶Suv39H2和Ezh2以及分泌蛋白Vmo1。母羊的先前数据显示,T3和雌二醇(E2),这两种关键激素都是季节性繁殖的正确时机,不同地影响MBH季节性转录组,并确定了这些激素可能起作用的细胞和分子靶标。相比之下,关于孕酮(P4)对MBH转录组的潜在影响的信息不存在.这里,我们证明,P4对母羊或母羊都没有明显的转录影响。一起来看,我们的数据表明,在MBH中,Dimes和母羊拥有一组共同的核心光周期响应基因,并最终证明P4不是MBH转录组的关键调节因子。
Photoperiod is the main environmental driver of seasonal responses in organisms living at temperate and polar latitudes. Other external cues such as food and temperature, and internal cues including hormones, intervene to fine-tune phasing of physiological functions to the solar year. In mammals, the medio-basal hypothalamus (MBH) is the key integrator of these cues, which orchestrates a wide array of seasonal functions, including breeding. Here, using RNAseq and RT-qPCR, we demonstrate that molecular components of the photoperiodic response previously identified in ewes are broadly conserved in does (female goats, Capra hircus), with a common core of ∼50 genes. This core group can be defined as the \"MBH seasonal trancriptome\", which includes key players of the pars tuberalis-tanycytes neuroendocrine retrograde pathway that governs intra-MBH photoperiodic switches of triiodothyronine (T3) production (Tshb, Eya3, Dio2 and SlcO1c1), the two histone methyltransferases Suv39H2 and Ezh2 and the secreted protein Vmo1. Prior data in ewes revealed that T3 and estradiol (E2), both key hormones for the proper timing of seasonal breeding, differentially impact the MBH seasonal transcriptome, and identified cellular and molecular targets through which these hormones might act. In contrast, information regarding the potential impact of progesterone (P4) upon the MBH transcriptome was nonexistent. Here, we demonstrate that P4 has no discernible transcriptional impact in either does or ewes. Taken together, our data show that does and ewes possess a common core set of photoperiod-responsive genes in the MBH and conclusively demonstrate that P4 is not a key regulator of the MBH transcriptome.