Panulirusargus病毒1(PaV1)是加勒比海多刺龙虾中描述的第一个也是唯一的自然致病病毒,帕努利鲁·阿格斯。一般与argus合发的足足动物中的PaV1感染,包括斑点多刺龙虾Panulirusguttatus,以前没有描述过。2016年,在SummerlandKey附近收集了14只加勒比海和5只斑点多刺龙虾,佛罗里达,补充新奥尔良美洲奥杜邦水族馆的常住人口,路易斯安那州。隔离5个月后,加勒比海和斑点多刺龙虾开始表现出嗜睡和在蜕皮中死亡的临床迹象。最初的组织学评估显示,表皮海绵状结缔组织的循环血细胞中存在核内包涵体,提示是病毒感染.使用实时定量聚合酶链反应(qPCR),来自已故加勒比海和斑点多刺龙虾的肝胰腺和血淋巴样本对白斑综合征病毒呈阴性,对PaV1呈阳性。核内,嗜酸性到两性,主要在新鲜安乐死的加勒比多刺龙虾的肝胰腺中的固定吞噬细胞和循环血细胞中观察到的CowdryA型包涵体与PaV1感染一致。透射电镜显示与肝胰小管相关的血细胞含有病毒包涵体,尺寸,和形态与先前描述的PaV1感染一致。这些发现强调了在多刺龙虾中Pav1的研究和诊断中,结合组织病理学和电子显微镜使用分子诊断的重要性。需要进一步研究以调查斑点多刺龙虾中PaV1相关死亡事件与微观病变的关系。
Panulirus argus virus 1 (PaV1) is the first and only naturally occurring pathogenic virus described in the Caribbean spiny lobster, Panulirus argus. PaV1 infection in decapod species that commonly co-occur with P. argus, including the spotted spiny lobster Panulirus guttatus, has not been previously described. In 2016, 14 Caribbean and 5 spotted spiny lobsters were collected near Summerland Key, Florida, to supplement the resident population of the Audubon Aquarium of the Americas in New Orleans, Louisiana. After 5 months in quarantine, Caribbean and spotted spiny lobsters began to exhibit clinical signs of lethargy and dying in the molt. Initial histologic evaluation revealed intranuclear inclusion bodies in circulating hemocytes in the spongy connective tissue of the epidermis, suggesting a viral infection. Samples of hepatopancreas and hemolymph from deceased Caribbean and spotted spiny lobsters tested negative for white spot syndrome virus and positive for PaV1 using real-time quantitative polymerase chain reaction (qPCR). Intranuclear, eosinophilic to amphophilic, Cowdry type A inclusion bodies observed primarily within fixed phagocytes and circulating hemocytes in the hepatopancreas of freshly euthanized Caribbean spiny lobsters were consistent with PaV1 infection. Transmission electron microscopy revealed that hemocytes associated with hepatopancreatic tubules contained viral inclusions with location, size, and morphology consistent with previously described PaV1 infection. These findings highlight the significance of using molecular diagnostics in conjunction with histopathology and electron microscopy in the investigation and diagnosis of PaV1 in spiny lobsters. Further study is required to investigate the relationship of PaV1-associated mortality events and microscopic lesions in the spotted spiny lobster.