已提出介孔二氧化硅纳米粒子(MSN)的表面官能化作为增强基于MSN的载体平台的生物相容性和效率的有效策略。在这里,由聚鸟氨酸(PLO)和羧甲基香菇多糖(LC)组成的天然聚电解质多层通过逐层(LbL)自组装技术涂覆在MSN的表面,并以ζ电位为特征,FTIR,13CNMR,SEM,TEM,XRD,TG。当用聚电解质涂覆时,制备的载体呈现交替的正电势和负电势,与裸露的MSN相比,MSN-PLO/LC的表面更粗糙。生物相容性测试,包括细胞相容性,血液相容性,和组织相容性,结果表明,改性LC可以提高MSNs的生物相容性。将多柔比星(DOX)加载到制备的MSN-PLO/LC中,构建了高载量缓释给药系统,在人宫颈癌细胞系(Hela)中表现出明显的抗增殖作用。因此,基于MSN的PLO/LCLbLNP(一层一层自组装的PLO/LC层纳米颗粒),这很容易通过静电相互作用制备,可以被认为是未来人类宫颈癌治疗的有前途的药物化疗平台和递送技术。
Surface functionalization of mesoporous silica nanoparticles (MSNs) has been proposed as an efficient strategy for enhancing the biocompatibility and efficiency of an MSN-based carrier platform. Herein, natural polyelectrolyte multilayers composed of poly-l-ornithine (
PLO) and carboxymethyl lentinan (LC) were coated on the surface of MSNs through a layer-by-layer (LbL) self-assembly technique, and were characterized by ζ-potential, FTIR, 13C NMR, SEM, TEM, XRD, and TG. The prepared carrier presented alternating positive and negative potentials when coated with the polyelectrolytes, and the surface of MSN-
PLO/LC was rougher compared to the naked MSNs. The biocompatibility tests, including cytocompatibility, hemocompatibility, and histocompatibility, showed that MSNs biocompatibility could be improved by modifying LC. A high loading and sustained release drug delivery system was constructed after loading doxorubicin (DOX) into the prepared MSN-
PLO/LC, which exhibited significant anti-proliferative efficiency in human cervical cancer cell lines (Hela). Therefore, the
PLO/LC LbL NPs (layer-by-layer self-assembled nanoparticles coated with
PLO/LC layers) based on MSNs, which is easily prepared by electrostatic interactions, can be considered a promising drug chemotherapeutic platform and delivery technique for future human cervical cancer therapy.